Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02200 and PS417_02205 are separated by 138 nucleotides PS417_02205 and PS417_02210 are separated by 72 nucleotides PS417_02210 and PS417_02215 are separated by 315 nucleotides
PS417_02200: PS417_02200 - lactate dehydrogenase, at 495,965 to 496,801
_02200
PS417_02205: PS417_02205 - peptide methionine sulfoxide reductase, at 496,940 to 497,587
_02205
PS417_02210: PS417_02210 - diguanylate cyclase, at 497,660 to 500,353
_02210
PS417_02215: PS417_02215 - dihydrolipoamide acetyltransferase, at 500,669 to 502,315
_02215
Position (kb) 497
498
499
500
501 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 496.810 kb on + strand at 496.811 kb on - strand at 496.863 kb on - strand at 496.863 kb on - strand at 496.863 kb on - strand at 496.863 kb on - strand at 496.870 kb on + strand at 496.873 kb on + strand at 496.874 kb on - strand at 496.941 kb on + strand at 496.981 kb on - strand at 497.005 kb on - strand, within PS417_02205 at 497.005 kb on - strand, within PS417_02205 at 497.016 kb on + strand, within PS417_02205 at 497.031 kb on + strand, within PS417_02205 at 497.031 kb on + strand, within PS417_02205 at 497.101 kb on - strand, within PS417_02205 at 497.151 kb on + strand, within PS417_02205 at 497.152 kb on - strand, within PS417_02205 at 497.166 kb on + strand, within PS417_02205 at 497.269 kb on - strand, within PS417_02205 at 497.269 kb on - strand, within PS417_02205 at 497.269 kb on - strand, within PS417_02205 at 497.269 kb on - strand, within PS417_02205 at 497.322 kb on + strand, within PS417_02205 at 497.323 kb on - strand, within PS417_02205 at 497.822 kb on - strand at 497.941 kb on - strand, within PS417_02210 at 497.941 kb on - strand, within PS417_02210 at 499.038 kb on + strand, within PS417_02210 at 499.038 kb on + strand, within PS417_02210 at 499.252 kb on - strand, within PS417_02210 at 499.455 kb on - strand, within PS417_02210 at 499.983 kb on + strand, within PS417_02210 at 500.014 kb on + strand, within PS417_02210 at 500.015 kb on - strand, within PS417_02210 at 500.016 kb on + strand, within PS417_02210 at 500.016 kb on + strand, within PS417_02210 at 500.017 kb on - strand, within PS417_02210 at 500.161 kb on - strand at 500.257 kb on + strand at 500.258 kb on - strand at 500.472 kb on + strand at 500.472 kb on + strand at 500.472 kb on + strand at 500.473 kb on - strand at 500.474 kb on + strand at 500.475 kb on - strand at 500.475 kb on - strand at 500.528 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 496,810 + +0.1 496,811 - +0.5 496,863 - -1.5 496,863 - +0.7 496,863 - -0.4 496,863 - +0.4 496,870 + +1.0 496,873 + +0.2 496,874 - -0.1 496,941 + -1.1 496,981 - -3.9 497,005 - PS417_02205 0.10 +3.0 497,005 - PS417_02205 0.10 +0.1 497,016 + PS417_02205 0.12 +0.1 497,031 + PS417_02205 0.14 -1.4 497,031 + PS417_02205 0.14 -2.5 497,101 - PS417_02205 0.25 +2.1 497,151 + PS417_02205 0.33 +0.9 497,152 - PS417_02205 0.33 -0.9 497,166 + PS417_02205 0.35 -1.7 497,269 - PS417_02205 0.51 -2.1 497,269 - PS417_02205 0.51 -1.0 497,269 - PS417_02205 0.51 -0.2 497,269 - PS417_02205 0.51 +1.3 497,322 + PS417_02205 0.59 +0.4 497,323 - PS417_02205 0.59 -0.9 497,822 - -1.1 497,941 - PS417_02210 0.10 -2.5 497,941 - PS417_02210 0.10 +0.4 499,038 + PS417_02210 0.51 -4.4 499,038 + PS417_02210 0.51 -1.0 499,252 - PS417_02210 0.59 -4.1 499,455 - PS417_02210 0.67 -2.6 499,983 + PS417_02210 0.86 -0.6 500,014 + PS417_02210 0.87 -2.9 500,015 - PS417_02210 0.87 -3.0 500,016 + PS417_02210 0.87 -0.9 500,016 + PS417_02210 0.87 -5.1 500,017 - PS417_02210 0.87 -5.2 500,161 - -1.4 500,257 + -3.4 500,258 - -3.6 500,472 + +0.3 500,472 + -0.9 500,472 + -1.2 500,473 - +1.3 500,474 + -4.6 500,475 - +1.8 500,475 - +2.0 500,528 + -2.2
Or see this region's nucleotide sequence