Strain Fitness in Pseudomonas simiae WCS417 around PS417_21455

Experiment: JP1 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_21450 and PS417_21455 are separated by 34 nucleotidesPS417_21455 and PS417_21460 are separated by 13 nucleotidesPS417_21460 and PS417_21465 are separated by 105 nucleotides PS417_21450: PS417_21450 - phosphate acyltransferase, at 4,657,191 to 4,658,171 _21450 PS417_21455: PS417_21455 - 50S ribosomal protein L32, at 4,658,206 to 4,658,388 _21455 PS417_21460: PS417_21460 - metal-binding protein, at 4,658,402 to 4,658,932 _21460 PS417_21465: PS417_21465 - septum formation inhibitor Maf, at 4,659,038 to 4,659,616 _21465 Position (kb) 4658 4659Strain fitness (log2 ratio) -1 0 1 2at 4657.576 kb on + strand, within PS417_21450at 4657.880 kb on + strand, within PS417_21450at 4658.947 kb on - strandat 4659.048 kb on + strandat 4659.049 kb on - strandat 4659.052 kb on - strandat 4659.052 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction JP1 MOI 1
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4,657,576 + PS417_21450 0.39 +0.5
4,657,880 + PS417_21450 0.70 +0.8
4,658,947 - -0.3
4,659,048 + +1.9
4,659,049 - +0.3
4,659,052 - +0.7
4,659,052 - -1.1

Or see this region's nucleotide sequence