Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_19800 and PS417_19805 overlap by 8 nucleotides PS417_19805 and PS417_19810 are separated by 16 nucleotides PS417_19810 and PS417_19815 are separated by 131 nucleotides
PS417_19800: PS417_19800 - flagellar motor switch protein FliG, at 4,298,448 to 4,299,467
_19800
PS417_19805: PS417_19805 - flagellar M-ring protein FliF, at 4,299,460 to 4,301,238
_19805
PS417_19810: PS417_19810 - flagellar hook-basal body protein FliE, at 4,301,255 to 4,301,584
_19810
PS417_19815: PS417_19815 - Fis family transcriptional regulator, at 4,301,716 to 4,303,092
_19815
Position (kb)
4299
4300
4301
4302 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4299.220 kb on + strand at 4299.221 kb on - strand, within PS417_19800 at 4299.221 kb on - strand, within PS417_19800 at 4299.555 kb on - strand at 4299.800 kb on + strand, within PS417_19805 at 4300.702 kb on + strand, within PS417_19805 at 4301.244 kb on - strand at 4301.283 kb on + strand at 4301.303 kb on - strand, within PS417_19810 at 4301.303 kb on - strand, within PS417_19810 at 4301.634 kb on + strand at 4301.634 kb on + strand at 4301.635 kb on - strand at 4301.645 kb on + strand at 4301.718 kb on - strand at 4301.718 kb on - strand at 4301.728 kb on + strand at 4301.729 kb on - strand at 4301.729 kb on - strand at 4301.732 kb on + strand at 4301.732 kb on + strand at 4301.733 kb on - strand at 4301.733 kb on - strand at 4301.733 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 4,299,220 + -0.3 4,299,221 - PS417_19800 0.76 -1.0 4,299,221 - PS417_19800 0.76 -1.7 4,299,555 - -2.8 4,299,800 + PS417_19805 0.19 -3.3 4,300,702 + PS417_19805 0.70 -2.4 4,301,244 - -2.5 4,301,283 + -2.3 4,301,303 - PS417_19810 0.15 +0.3 4,301,303 - PS417_19810 0.15 +1.0 4,301,634 + -1.0 4,301,634 + -4.2 4,301,635 - +0.4 4,301,645 + -2.1 4,301,718 - +0.7 4,301,718 - +0.6 4,301,728 + -1.8 4,301,729 - -4.0 4,301,729 - +0.2 4,301,732 + -0.4 4,301,732 + -1.9 4,301,733 - +1.1 4,301,733 - -0.3 4,301,733 - -0.3
Or see this region's nucleotide sequence