Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_18770 and PS417_18775 are separated by 27 nucleotides PS417_18775 and PS417_18780 are separated by 8 nucleotides PS417_18780 and PS417_18790 are separated by 180 nucleotides
PS417_18770: PS417_18770 - hypothetical protein, at 4,071,080 to 4,072,063
_18770
PS417_18775: PS417_18775 - 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase, at 4,072,091 to 4,073,119
_18775
PS417_18780: PS417_18780 - flavin reductase, at 4,073,128 to 4,073,637
_18780
PS417_18790: PS417_18790 - amino acid transporter LysE, at 4,073,818 to 4,074,426
_18790
Position (kb)
4072
4073
4074 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4071.131 kb on + strand at 4071.437 kb on + strand, within PS417_18770 at 4071.537 kb on + strand, within PS417_18770 at 4071.581 kb on - strand, within PS417_18770 at 4071.701 kb on + strand, within PS417_18770 at 4071.701 kb on + strand, within PS417_18770 at 4071.748 kb on + strand at 4071.770 kb on + strand, within PS417_18770 at 4071.771 kb on - strand, within PS417_18770 at 4071.797 kb on + strand, within PS417_18770 at 4071.869 kb on + strand, within PS417_18770 at 4071.870 kb on - strand, within PS417_18770 at 4071.878 kb on - strand, within PS417_18770 at 4071.926 kb on + strand, within PS417_18770 at 4071.946 kb on - strand, within PS417_18770 at 4071.948 kb on - strand, within PS417_18770 at 4072.070 kb on + strand at 4072.108 kb on + strand at 4072.109 kb on - strand at 4072.133 kb on - strand at 4072.514 kb on + strand, within PS417_18775 at 4072.528 kb on + strand, within PS417_18775 at 4072.528 kb on + strand, within PS417_18775 at 4072.703 kb on - strand, within PS417_18775 at 4072.793 kb on + strand, within PS417_18775 at 4072.856 kb on - strand, within PS417_18775 at 4072.856 kb on - strand, within PS417_18775 at 4073.258 kb on - strand, within PS417_18780 at 4073.318 kb on - strand, within PS417_18780 at 4073.749 kb on - strand at 4073.749 kb on - strand at 4073.874 kb on + strand at 4073.874 kb on + strand at 4073.875 kb on - strand at 4073.940 kb on - strand, within PS417_18790 at 4073.940 kb on - strand, within PS417_18790 at 4073.966 kb on + strand, within PS417_18790 at 4073.966 kb on + strand, within PS417_18790 at 4073.966 kb on + strand, within PS417_18790 at 4073.967 kb on - strand, within PS417_18790 at 4073.967 kb on - strand, within PS417_18790 at 4073.967 kb on - strand, within PS417_18790
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 4,071,131 + +0.5 4,071,437 + PS417_18770 0.36 -0.8 4,071,537 + PS417_18770 0.46 +0.2 4,071,581 - PS417_18770 0.51 -2.6 4,071,701 + PS417_18770 0.63 +0.8 4,071,701 + PS417_18770 0.63 +1.7 4,071,748 + +0.4 4,071,770 + PS417_18770 0.70 -0.2 4,071,771 - PS417_18770 0.70 +0.7 4,071,797 + PS417_18770 0.73 -1.4 4,071,869 + PS417_18770 0.80 -0.7 4,071,870 - PS417_18770 0.80 -0.7 4,071,878 - PS417_18770 0.81 -0.4 4,071,926 + PS417_18770 0.86 +1.8 4,071,946 - PS417_18770 0.88 +3.4 4,071,948 - PS417_18770 0.88 -1.4 4,072,070 + -0.1 4,072,108 + +0.9 4,072,109 - -0.2 4,072,133 - +2.4 4,072,514 + PS417_18775 0.41 +2.3 4,072,528 + PS417_18775 0.42 +0.4 4,072,528 + PS417_18775 0.42 +1.0 4,072,703 - PS417_18775 0.59 -0.4 4,072,793 + PS417_18775 0.68 +0.2 4,072,856 - PS417_18775 0.74 -0.6 4,072,856 - PS417_18775 0.74 -2.1 4,073,258 - PS417_18780 0.25 -1.4 4,073,318 - PS417_18780 0.37 -0.4 4,073,749 - +1.4 4,073,749 - +0.0 4,073,874 + +0.8 4,073,874 + +0.3 4,073,875 - +1.9 4,073,940 - PS417_18790 0.20 +0.0 4,073,940 - PS417_18790 0.20 -0.5 4,073,966 + PS417_18790 0.24 -0.9 4,073,966 + PS417_18790 0.24 +0.4 4,073,966 + PS417_18790 0.24 +0.3 4,073,967 - PS417_18790 0.24 -1.6 4,073,967 - PS417_18790 0.24 -1.4 4,073,967 - PS417_18790 0.24 +0.4
Or see this region's nucleotide sequence