Strain Fitness in Pseudomonas simiae WCS417 around PS417_01835

Experiment: JP1 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_01825 and PS417_01830 are separated by 87 nucleotidesPS417_01830 and PS417_01835 overlap by 4 nucleotidesPS417_01835 and PS417_01840 overlap by 4 nucleotidesPS417_01840 and PS417_01845 are separated by 10 nucleotidesPS417_01845 and PS417_01850 are separated by 26 nucleotides PS417_01825: PS417_01825 - chemotaxis protein, at 407,805 to 409,721 _01825 PS417_01830: PS417_01830 - 16S rRNA methyltransferase, at 409,809 to 410,516 _01830 PS417_01835: PS417_01835 - twin-arginine protein translocation system subunit TatC, at 410,513 to 411,301 _01835 PS417_01840: PS417_01840 - preprotein translocase, at 411,298 to 411,702 _01840 PS417_01845: PS417_01845 - preprotein translocase subunit SecA, at 411,713 to 411,991 _01845 PS417_01850: PS417_01850 - phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31) (from data), at 412,018 to 412,350 _01850 Position (kb) 410 411 412Strain fitness (log2 ratio) -2 -1 0 1 2at 409.633 kb on - strandat 409.644 kb on + strandat 409.645 kb on - strandat 409.645 kb on - strandat 409.789 kb on + strandat 410.189 kb on - strand, within PS417_01830at 411.322 kb on - strandat 411.767 kb on - strand, within PS417_01845at 412.141 kb on + strand, within PS417_01850at 412.142 kb on - strand, within PS417_01850at 412.142 kb on - strand, within PS417_01850at 412.280 kb on + strand, within PS417_01850at 412.280 kb on + strand, within PS417_01850at 412.281 kb on - strand, within PS417_01850

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Per-strain Table

Position Strand Gene LocusTag Fraction JP1 MOI 1
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409,633 - +0.2
409,644 + -1.3
409,645 - +0.8
409,645 - -0.3
409,789 + +1.3
410,189 - PS417_01830 0.54 -0.2
411,322 - +0.7
411,767 - PS417_01845 0.19 +2.5
412,141 + PS417_01850 0.37 -1.3
412,142 - PS417_01850 0.37 -2.0
412,142 - PS417_01850 0.37 -1.6
412,280 + PS417_01850 0.79 +1.1
412,280 + PS417_01850 0.79 -0.2
412,281 - PS417_01850 0.79 +0.7

Or see this region's nucleotide sequence