Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01825 and PS417_01830 are separated by 87 nucleotides PS417_01830 and PS417_01835 overlap by 4 nucleotides PS417_01835 and PS417_01840 overlap by 4 nucleotides
PS417_01825: PS417_01825 - chemotaxis protein, at 407,805 to 409,721
_01825
PS417_01830: PS417_01830 - 16S rRNA methyltransferase, at 409,809 to 410,516
_01830
PS417_01835: PS417_01835 - twin-arginine protein translocation system subunit TatC, at 410,513 to 411,301
_01835
PS417_01840: PS417_01840 - preprotein translocase, at 411,298 to 411,702
_01840
Position (kb) 409
410
411 Strain fitness (log2 ratio) -1
0
1
2 at 409.156 kb on + strand, within PS417_01825 at 409.156 kb on + strand, within PS417_01825 at 409.364 kb on + strand, within PS417_01825 at 409.365 kb on - strand, within PS417_01825 at 409.365 kb on - strand, within PS417_01825 at 409.633 kb on - strand at 409.644 kb on + strand at 409.645 kb on - strand at 409.645 kb on - strand at 409.789 kb on + strand at 410.189 kb on - strand, within PS417_01830 at 411.322 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 409,156 + PS417_01825 0.70 +1.4 409,156 + PS417_01825 0.70 +2.0 409,364 + PS417_01825 0.81 +2.4 409,365 - PS417_01825 0.81 -0.7 409,365 - PS417_01825 0.81 -0.6 409,633 - +0.2 409,644 + -1.3 409,645 - +0.8 409,645 - -0.3 409,789 + +1.3 410,189 - PS417_01830 0.54 -0.2 411,322 - +0.7
Or see this region's nucleotide sequence