Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_17020 and PS417_17025 are separated by 61 nucleotides PS417_17025 and PS417_17030 overlap by 4 nucleotides PS417_17030 and PS417_17035 overlap by 4 nucleotides PS417_17035 and PS417_17040 are separated by 131 nucleotides
PS417_17020: PS417_17020 - methylcrotonoyl-CoA carboxylase, at 3,718,747 to 3,720,354
_17020
PS417_17025: PS417_17025 - gamma-carboxygeranoyl-CoA hydratase, at 3,720,416 to 3,721,222
_17025
PS417_17030: PS417_17030 - 3-methylcrotonyl-CoA carboxylase, at 3,721,219 to 3,723,144
_17030
PS417_17035: PS417_17035 - Cro/Cl family transcriptional regulator, at 3,723,141 to 3,723,773
_17035
PS417_17040: PS417_17040 - hypothetical protein, at 3,723,905 to 3,724,255
_17040
Position (kb)
3721
3722
3723
3724 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3720.352 kb on + strand at 3720.352 kb on + strand at 3720.395 kb on + strand at 3720.395 kb on + strand at 3720.396 kb on - strand at 3722.804 kb on + strand, within PS417_17030 at 3723.466 kb on + strand, within PS417_17035 at 3723.467 kb on - strand at 3723.785 kb on + strand at 3723.821 kb on - strand at 3723.824 kb on - strand at 3723.844 kb on + strand at 3723.844 kb on + strand at 3723.853 kb on + strand at 3723.853 kb on + strand at 3723.853 kb on + strand at 3723.854 kb on - strand at 3723.856 kb on + strand at 3723.857 kb on - strand at 3723.857 kb on - strand at 3723.868 kb on + strand at 3723.869 kb on - strand at 3723.894 kb on + strand at 3723.894 kb on + strand at 3723.920 kb on + strand at 3723.963 kb on + strand, within PS417_17040 at 3723.963 kb on + strand, within PS417_17040 at 3724.002 kb on - strand, within PS417_17040 at 3724.002 kb on - strand, within PS417_17040 at 3724.002 kb on - strand, within PS417_17040 at 3724.127 kb on + strand, within PS417_17040
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 3,720,352 + -3.1 3,720,352 + -0.0 3,720,395 + +1.3 3,720,395 + -1.7 3,720,396 - +1.9 3,722,804 + PS417_17030 0.82 -0.6 3,723,466 + PS417_17035 0.51 -0.6 3,723,467 - +1.9 3,723,785 + -2.2 3,723,821 - +1.5 3,723,824 - -0.8 3,723,844 + +1.3 3,723,844 + -0.1 3,723,853 + -0.3 3,723,853 + -0.0 3,723,853 + +2.3 3,723,854 - +1.3 3,723,856 + -2.5 3,723,857 - -0.3 3,723,857 - +0.1 3,723,868 + -1.2 3,723,869 - -0.5 3,723,894 + +0.7 3,723,894 + -1.7 3,723,920 + -0.7 3,723,963 + PS417_17040 0.17 -1.3 3,723,963 + PS417_17040 0.17 -0.6 3,724,002 - PS417_17040 0.28 -0.4 3,724,002 - PS417_17040 0.28 -0.8 3,724,002 - PS417_17040 0.28 +1.0 3,724,127 + PS417_17040 0.63 -0.2
Or see this region's nucleotide sequence