Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_16995 and PS417_17000 are separated by 80 nucleotides PS417_17000 and PS417_17005 are separated by 16 nucleotides
PS417_16995: PS417_16995 - LysR family transcriptional regulator, at 3,712,780 to 3,713,715
_16995
PS417_17000: PS417_17000 - MerR family transcriptional regulator, at 3,713,796 to 3,714,188
_17000
PS417_17005: PS417_17005 - hydroxymethylglutaryl-CoA lyase, at 3,714,205 to 3,715,104
_17005
Position (kb)
3713
3714
3715 Strain fitness (log2 ratio)
-2
-1
0
1 at 3712.831 kb on + strand at 3712.832 kb on - strand at 3713.377 kb on - strand, within PS417_16995 at 3713.377 kb on - strand, within PS417_16995 at 3713.467 kb on + strand, within PS417_16995 at 3713.468 kb on - strand, within PS417_16995 at 3713.599 kb on + strand, within PS417_16995 at 3713.600 kb on - strand, within PS417_16995 at 3713.600 kb on - strand, within PS417_16995 at 3713.713 kb on + strand at 3713.805 kb on - strand at 3713.808 kb on + strand at 3713.808 kb on + strand at 3713.809 kb on - strand at 3713.865 kb on + strand, within PS417_17000 at 3713.916 kb on + strand, within PS417_17000 at 3715.107 kb on - strand at 3715.163 kb on + strand at 3715.163 kb on + strand at 3715.163 kb on + strand at 3715.163 kb on + strand at 3715.163 kb on + strand at 3715.164 kb on - strand at 3715.173 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 3,712,831 + +0.7 3,712,832 - -0.1 3,713,377 - PS417_16995 0.64 -0.7 3,713,377 - PS417_16995 0.64 -1.2 3,713,467 + PS417_16995 0.73 +0.8 3,713,468 - PS417_16995 0.74 +0.2 3,713,599 + PS417_16995 0.88 -0.3 3,713,600 - PS417_16995 0.88 -1.0 3,713,600 - PS417_16995 0.88 -1.5 3,713,713 + -0.1 3,713,805 - -1.3 3,713,808 + +1.3 3,713,808 + +1.3 3,713,809 - +0.3 3,713,865 + PS417_17000 0.18 -2.6 3,713,916 + PS417_17000 0.31 -2.6 3,715,107 - +0.8 3,715,163 + +0.7 3,715,163 + +0.3 3,715,163 + -0.0 3,715,163 + +0.8 3,715,163 + +0.3 3,715,164 - -0.6 3,715,173 + +0.8
Or see this region's nucleotide sequence