Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_16045 and PS417_16050 are separated by 205 nucleotides PS417_16050 and PS417_16055 overlap by 4 nucleotides PS417_16055 and PS417_16060 are separated by 15 nucleotides
PS417_16045: PS417_16045 - acyl-CoA dehydrogenase, at 3,516,279 to 3,517,457
_16045
PS417_16050: PS417_16050 - energy transducer TonB, at 3,517,663 to 3,518,436
_16050
PS417_16055: PS417_16055 - biopolymer transporter TolR, at 3,518,433 to 3,518,879
_16055
PS417_16060: PS417_16060 - protein tolQ, at 3,518,895 to 3,519,587
_16060
Position (kb)
3517
3518
3519 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3516.765 kb on + strand, within PS417_16045 at 3517.050 kb on + strand, within PS417_16045 at 3517.258 kb on - strand, within PS417_16045 at 3517.334 kb on + strand, within PS417_16045 at 3517.403 kb on - strand at 3517.452 kb on - strand at 3517.452 kb on - strand at 3517.526 kb on + strand at 3517.559 kb on - strand at 3517.664 kb on + strand at 3517.664 kb on + strand at 3518.243 kb on - strand, within PS417_16050 at 3518.256 kb on + strand, within PS417_16050 at 3518.470 kb on - strand at 3518.542 kb on + strand, within PS417_16055 at 3518.542 kb on + strand, within PS417_16055 at 3518.543 kb on - strand, within PS417_16055 at 3518.975 kb on - strand, within PS417_16060 at 3519.178 kb on + strand, within PS417_16060 at 3519.178 kb on + strand at 3519.179 kb on - strand, within PS417_16060 at 3519.313 kb on + strand, within PS417_16060
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 3,516,765 + PS417_16045 0.41 -1.5 3,517,050 + PS417_16045 0.65 +0.7 3,517,258 - PS417_16045 0.83 -1.1 3,517,334 + PS417_16045 0.89 +1.5 3,517,403 - -0.3 3,517,452 - +1.9 3,517,452 - -0.3 3,517,526 + -0.4 3,517,559 - +0.6 3,517,664 + -0.6 3,517,664 + +1.9 3,518,243 - PS417_16050 0.75 -0.8 3,518,256 + PS417_16050 0.77 +0.8 3,518,470 - +1.9 3,518,542 + PS417_16055 0.24 +2.9 3,518,542 + PS417_16055 0.24 +0.2 3,518,543 - PS417_16055 0.25 +2.0 3,518,975 - PS417_16060 0.12 +0.5 3,519,178 + PS417_16060 0.41 -1.1 3,519,178 + -0.3 3,519,179 - PS417_16060 0.41 +0.6 3,519,313 + PS417_16060 0.60 -2.9
Or see this region's nucleotide sequence