Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_15245 and PS417_15250 are separated by 333 nucleotides PS417_15250 and PS417_15255 are separated by 4 nucleotides PS417_15255 and PS417_15260 overlap by 4 nucleotides
PS417_15245: PS417_15245 - PTS sugar transporter subunit IIC, at 3,342,887 to 3,344,962
_15245
PS417_15250: PS417_15250 - DNA repair protein, at 3,345,296 to 3,345,625
_15250
PS417_15255: PS417_15255 - serine/threonine protein kinase, at 3,345,630 to 3,346,475
_15255
PS417_15260: PS417_15260 - protein phosphatase, at 3,346,472 to 3,347,185
_15260
Position (kb)
3345
3346 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3344.372 kb on + strand, within PS417_15245 at 3344.372 kb on + strand, within PS417_15245 at 3344.373 kb on - strand, within PS417_15245 at 3344.373 kb on - strand, within PS417_15245 at 3344.373 kb on - strand, within PS417_15245 at 3344.373 kb on - strand, within PS417_15245 at 3344.470 kb on + strand, within PS417_15245 at 3344.648 kb on + strand, within PS417_15245 at 3344.649 kb on - strand, within PS417_15245 at 3344.649 kb on - strand, within PS417_15245 at 3345.182 kb on + strand at 3345.201 kb on + strand at 3345.289 kb on + strand at 3345.564 kb on + strand, within PS417_15250 at 3345.596 kb on + strand at 3345.646 kb on - strand at 3345.646 kb on - strand at 3345.646 kb on - strand at 3345.844 kb on + strand, within PS417_15255 at 3345.845 kb on - strand, within PS417_15255 at 3345.845 kb on - strand, within PS417_15255 at 3346.178 kb on + strand, within PS417_15255 at 3346.609 kb on - strand, within PS417_15260
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 3,344,372 + PS417_15245 0.72 -1.1 3,344,372 + PS417_15245 0.72 -1.7 3,344,373 - PS417_15245 0.72 +1.3 3,344,373 - PS417_15245 0.72 +0.9 3,344,373 - PS417_15245 0.72 +0.7 3,344,373 - PS417_15245 0.72 +0.0 3,344,470 + PS417_15245 0.76 -0.2 3,344,648 + PS417_15245 0.85 -0.2 3,344,649 - PS417_15245 0.85 -1.6 3,344,649 - PS417_15245 0.85 +0.8 3,345,182 + +0.2 3,345,201 + -0.7 3,345,289 + -0.3 3,345,564 + PS417_15250 0.81 +0.2 3,345,596 + +0.8 3,345,646 - +2.1 3,345,646 - +2.6 3,345,646 - +2.4 3,345,844 + PS417_15255 0.25 -0.5 3,345,845 - PS417_15255 0.25 +3.2 3,345,845 - PS417_15255 0.25 +2.5 3,346,178 + PS417_15255 0.65 -0.3 3,346,609 - PS417_15260 0.19 +1.4
Or see this region's nucleotide sequence