Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_15120 and PS417_15125 overlap by 4 nucleotides PS417_15125 and PS417_15135 are separated by 281 nucleotides PS417_15135 and PS417_15140 are separated by 156 nucleotides
PS417_15120: PS417_15120 - chromosome segregation protein SMC, at 3,307,470 to 3,311,108
_15120
PS417_15125: PS417_15125 - glutathione S-transferase, at 3,311,105 to 3,311,698
_15125
PS417_15135: PS417_15135 - hypothetical protein, at 3,311,980 to 3,312,159
_15135
PS417_15140: PS417_15140 - 3-carboxymuconate cyclase, at 3,312,316 to 3,313,491
_15140
Position (kb)
3311
3312
3313 Strain fitness (log2 ratio)
-1
0
1
2 at 3311.026 kb on + strand at 3311.123 kb on + strand at 3311.123 kb on + strand at 3311.124 kb on - strand at 3311.349 kb on + strand, within PS417_15125 at 3311.409 kb on - strand, within PS417_15125 at 3311.526 kb on - strand, within PS417_15125 at 3311.696 kb on + strand at 3311.784 kb on - strand at 3312.047 kb on + strand, within PS417_15135 at 3312.048 kb on - strand, within PS417_15135 at 3312.267 kb on + strand at 3312.268 kb on - strand at 3312.590 kb on + strand, within PS417_15140 at 3312.591 kb on - strand, within PS417_15140 at 3312.861 kb on - strand at 3312.909 kb on - strand, within PS417_15140 at 3313.012 kb on + strand, within PS417_15140 at 3313.077 kb on - strand, within PS417_15140 at 3313.077 kb on - strand, within PS417_15140
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 3,311,026 + +0.0 3,311,123 + +1.3 3,311,123 + +0.5 3,311,124 - -0.7 3,311,349 + PS417_15125 0.41 +1.1 3,311,409 - PS417_15125 0.51 +0.2 3,311,526 - PS417_15125 0.71 -0.7 3,311,696 + -0.2 3,311,784 - +2.2 3,312,047 + PS417_15135 0.37 -1.1 3,312,048 - PS417_15135 0.38 -1.1 3,312,267 + +0.5 3,312,268 - -0.4 3,312,590 + PS417_15140 0.23 +0.8 3,312,591 - PS417_15140 0.23 +0.0 3,312,861 - +1.3 3,312,909 - PS417_15140 0.50 -0.5 3,313,012 + PS417_15140 0.59 +0.1 3,313,077 - PS417_15140 0.65 +1.0 3,313,077 - PS417_15140 0.65 +0.2
Or see this region's nucleotide sequence