Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_14295 and PS417_14300 are separated by 187 nucleotides PS417_14300 and PS417_14305 are separated by 14 nucleotides PS417_14305 and PS417_14310 are separated by 42 nucleotides
PS417_14295: PS417_14295 - AraC family transcriptional regulator, at 3,128,638 to 3,129,396
_14295
PS417_14300: PS417_14300 - aspartate:proton symporter, at 3,129,584 to 3,131,203
_14300
PS417_14305: PS417_14305 - hydroxyproline-2-epimerase, at 3,131,218 to 3,132,144
_14305
PS417_14310: PS417_14310 - dihydrodipicolinate synthetase, at 3,132,187 to 3,133,134
_14310
Position (kb)
3129
3130
3131
3132 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3129.026 kb on + strand, within PS417_14295 at 3129.027 kb on - strand, within PS417_14295 at 3129.313 kb on + strand, within PS417_14295 at 3129.314 kb on - strand, within PS417_14295 at 3129.334 kb on + strand at 3129.334 kb on + strand at 3129.335 kb on - strand at 3129.538 kb on + strand at 3129.583 kb on + strand at 3129.583 kb on + strand at 3129.583 kb on + strand at 3129.767 kb on + strand, within PS417_14300 at 3129.768 kb on - strand, within PS417_14300 at 3129.921 kb on - strand, within PS417_14300 at 3130.075 kb on - strand, within PS417_14300 at 3130.217 kb on + strand, within PS417_14300 at 3130.323 kb on - strand, within PS417_14300 at 3130.458 kb on - strand, within PS417_14300 at 3130.509 kb on - strand, within PS417_14300 at 3130.509 kb on - strand, within PS417_14300 at 3130.509 kb on - strand, within PS417_14300 at 3130.509 kb on - strand, within PS417_14300 at 3130.536 kb on - strand, within PS417_14300 at 3130.536 kb on - strand, within PS417_14300 at 3130.536 kb on - strand at 3130.899 kb on - strand, within PS417_14300 at 3131.058 kb on + strand at 3131.087 kb on + strand at 3131.390 kb on + strand, within PS417_14305 at 3131.407 kb on + strand at 3131.677 kb on + strand, within PS417_14305 at 3131.908 kb on + strand at 3132.034 kb on + strand, within PS417_14305 at 3132.082 kb on + strand at 3132.083 kb on - strand at 3132.160 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 3,129,026 + PS417_14295 0.51 +0.1 3,129,027 - PS417_14295 0.51 -0.3 3,129,313 + PS417_14295 0.89 -0.5 3,129,314 - PS417_14295 0.89 -0.0 3,129,334 + -1.6 3,129,334 + -0.3 3,129,335 - +2.3 3,129,538 + -0.6 3,129,583 + +0.4 3,129,583 + -0.7 3,129,583 + -0.2 3,129,767 + PS417_14300 0.11 +1.0 3,129,768 - PS417_14300 0.11 +0.4 3,129,921 - PS417_14300 0.21 +0.1 3,130,075 - PS417_14300 0.30 -1.3 3,130,217 + PS417_14300 0.39 -0.7 3,130,323 - PS417_14300 0.46 +0.4 3,130,458 - PS417_14300 0.54 +0.1 3,130,509 - PS417_14300 0.57 -2.6 3,130,509 - PS417_14300 0.57 +0.7 3,130,509 - PS417_14300 0.57 +1.8 3,130,509 - PS417_14300 0.57 -2.8 3,130,536 - PS417_14300 0.59 -0.1 3,130,536 - PS417_14300 0.59 +1.3 3,130,536 - -0.3 3,130,899 - PS417_14300 0.81 +1.9 3,131,058 + -0.7 3,131,087 + -0.7 3,131,390 + PS417_14305 0.19 +1.7 3,131,407 + +0.3 3,131,677 + PS417_14305 0.50 -2.4 3,131,908 + -1.3 3,132,034 + PS417_14305 0.88 -0.0 3,132,082 + -0.2 3,132,083 - +2.1 3,132,160 + +0.3
Or see this region's nucleotide sequence