Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_13850 and PS417_13855 are separated by 9 nucleotides PS417_13855 and PS417_13860 are separated by 12 nucleotides PS417_13860 and PS417_13865 are separated by 200 nucleotides
PS417_13850: PS417_13850 - 2-methylbutanoyl-CoA dehydrogenase (EC 1.3.8.5) (from data) , at 3,042,450 to 3,043,577
_13850
PS417_13855: PS417_13855 - acetyl-CoA acetyltransferase, at 3,043,587 to 3,044,771
_13855
PS417_13860: PS417_13860 - 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, at 3,044,784 to 3,045,551
_13860
PS417_13865: PS417_13865 - bestrophin, at 3,045,752 to 3,046,645
_13865
Position (kb)
3043
3044
3045 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3043.006 kb on + strand at 3043.137 kb on + strand, within PS417_13850 at 3043.138 kb on - strand, within PS417_13850 at 3043.141 kb on + strand, within PS417_13850 at 3043.141 kb on + strand, within PS417_13850 at 3043.142 kb on - strand, within PS417_13850 at 3043.142 kb on - strand, within PS417_13850 at 3043.178 kb on + strand, within PS417_13850 at 3043.378 kb on + strand, within PS417_13850 at 3043.591 kb on + strand at 3043.592 kb on - strand at 3044.401 kb on + strand, within PS417_13855 at 3044.785 kb on + strand at 3044.785 kb on + strand at 3044.786 kb on - strand at 3044.786 kb on - strand at 3045.555 kb on + strand at 3045.556 kb on - strand at 3045.756 kb on + strand at 3045.757 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 3,043,006 + -0.7 3,043,137 + PS417_13850 0.61 +1.9 3,043,138 - PS417_13850 0.61 +0.1 3,043,141 + PS417_13850 0.61 -0.6 3,043,141 + PS417_13850 0.61 +0.6 3,043,142 - PS417_13850 0.61 -0.2 3,043,142 - PS417_13850 0.61 +1.3 3,043,178 + PS417_13850 0.65 +1.0 3,043,378 + PS417_13850 0.82 -0.5 3,043,591 + +0.9 3,043,592 - +0.6 3,044,401 + PS417_13855 0.69 -2.1 3,044,785 + -1.6 3,044,785 + -0.0 3,044,786 - +1.0 3,044,786 - +0.2 3,045,555 + -3.5 3,045,556 - -0.7 3,045,756 + +0.2 3,045,757 - +0.3
Or see this region's nucleotide sequence