Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_13565 and PS417_13575 are separated by 585 nucleotides PS417_13575 and PS417_13580 overlap by 39 nucleotides PS417_13580 and PS417_13585 are separated by 143 nucleotides
PS417_13565: PS417_13565 - permease, at 2,988,581 to 2,989,579
_13565
PS417_13575: PS417_13575 - succinylglutamate desuccinylase, at 2,990,165 to 2,991,163
_13575
PS417_13580: PS417_13580 - AraC family transcriptional regulator, at 2,991,125 to 2,991,982
_13580
PS417_13585: PS417_13585 - alpha/beta hydrolase, at 2,992,126 to 2,992,926
_13585
Position (kb)
2990
2991
2992 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2989.304 kb on + strand, within PS417_13565 at 2989.675 kb on - strand at 2989.999 kb on + strand at 2990.142 kb on - strand at 2990.149 kb on + strand at 2990.310 kb on + strand, within PS417_13575 at 2990.374 kb on + strand, within PS417_13575 at 2990.609 kb on + strand at 2990.610 kb on - strand, within PS417_13575 at 2990.903 kb on - strand, within PS417_13575 at 2991.138 kb on - strand at 2991.331 kb on - strand, within PS417_13580 at 2991.868 kb on - strand, within PS417_13580 at 2991.994 kb on + strand at 2992.001 kb on + strand at 2992.001 kb on + strand at 2992.002 kb on - strand at 2992.029 kb on - strand at 2992.056 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 2,989,304 + PS417_13565 0.72 +0.1 2,989,675 - +1.3 2,989,999 + -1.0 2,990,142 - -1.8 2,990,149 + +1.6 2,990,310 + PS417_13575 0.15 -0.6 2,990,374 + PS417_13575 0.21 -0.3 2,990,609 + +1.9 2,990,610 - PS417_13575 0.45 -0.6 2,990,903 - PS417_13575 0.74 -1.0 2,991,138 - +0.0 2,991,331 - PS417_13580 0.24 +0.4 2,991,868 - PS417_13580 0.87 +0.0 2,991,994 + -0.7 2,992,001 + +0.1 2,992,001 + -0.5 2,992,002 - -0.8 2,992,029 - +0.6 2,992,056 + -1.4
Or see this region's nucleotide sequence