Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12455 and PS417_12460 are separated by 66 nucleotides PS417_12460 and PS417_12465 are separated by 342 nucleotides PS417_12465 and PS417_12470 are separated by 108 nucleotides
PS417_12455: PS417_12455 - hydrolase, at 2,709,313 to 2,710,137
_12455
PS417_12460: PS417_12460 - hypothetical protein, at 2,710,204 to 2,710,464
_12460
PS417_12465: PS417_12465 - Fis family transcriptional regulator, at 2,710,807 to 2,712,132
_12465
PS417_12470: PS417_12470 - hypothetical protein, at 2,712,241 to 2,713,542
_12470
Position (kb)
2710
2711
2712
2713 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2709.877 kb on + strand, within PS417_12455 at 2710.080 kb on + strand at 2710.081 kb on - strand at 2710.119 kb on + strand at 2710.151 kb on + strand at 2710.206 kb on - strand at 2710.206 kb on - strand at 2710.209 kb on + strand at 2710.210 kb on - strand at 2710.300 kb on - strand, within PS417_12460 at 2710.301 kb on + strand, within PS417_12460 at 2710.301 kb on + strand, within PS417_12460 at 2710.302 kb on - strand, within PS417_12460 at 2710.302 kb on - strand, within PS417_12460 at 2710.302 kb on - strand, within PS417_12460 at 2710.302 kb on - strand, within PS417_12460 at 2710.326 kb on + strand, within PS417_12460 at 2710.528 kb on + strand at 2710.529 kb on - strand at 2710.534 kb on + strand at 2710.535 kb on - strand at 2710.698 kb on - strand at 2710.700 kb on - strand at 2710.761 kb on + strand at 2710.762 kb on - strand at 2710.973 kb on - strand at 2711.057 kb on + strand, within PS417_12465 at 2711.110 kb on - strand, within PS417_12465 at 2711.189 kb on - strand, within PS417_12465 at 2711.544 kb on - strand, within PS417_12465 at 2711.544 kb on - strand, within PS417_12465 at 2711.693 kb on - strand, within PS417_12465 at 2711.693 kb on - strand, within PS417_12465 at 2711.753 kb on + strand, within PS417_12465 at 2711.753 kb on + strand, within PS417_12465 at 2711.753 kb on + strand, within PS417_12465 at 2711.786 kb on - strand, within PS417_12465 at 2711.927 kb on + strand, within PS417_12465 at 2711.927 kb on + strand, within PS417_12465 at 2711.927 kb on + strand, within PS417_12465 at 2711.927 kb on + strand, within PS417_12465 at 2711.928 kb on - strand, within PS417_12465 at 2711.928 kb on - strand, within PS417_12465 at 2711.928 kb on - strand, within PS417_12465 at 2711.983 kb on - strand, within PS417_12465 at 2712.249 kb on - strand at 2712.249 kb on - strand at 2712.365 kb on + strand at 2712.465 kb on + strand at 2712.493 kb on + strand, within PS417_12470 at 2712.591 kb on + strand, within PS417_12470 at 2712.599 kb on + strand, within PS417_12470 at 2712.704 kb on + strand, within PS417_12470 at 2712.704 kb on + strand, within PS417_12470 at 2712.705 kb on - strand, within PS417_12470 at 2712.705 kb on - strand, within PS417_12470 at 2712.705 kb on - strand, within PS417_12470 at 2712.859 kb on - strand, within PS417_12470 at 2712.923 kb on + strand, within PS417_12470 at 2712.987 kb on - strand, within PS417_12470 at 2713.041 kb on + strand, within PS417_12470 at 2713.056 kb on + strand, within PS417_12470 at 2713.056 kb on + strand, within PS417_12470 at 2713.057 kb on - strand, within PS417_12470 at 2713.112 kb on + strand, within PS417_12470 at 2713.112 kb on + strand, within PS417_12470
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 2,709,877 + PS417_12455 0.68 -2.6 2,710,080 + -0.4 2,710,081 - +0.3 2,710,119 + +0.6 2,710,151 + +0.4 2,710,206 - -2.3 2,710,206 - -0.5 2,710,209 + -0.7 2,710,210 - -0.4 2,710,300 - PS417_12460 0.37 -0.9 2,710,301 + PS417_12460 0.37 +0.8 2,710,301 + PS417_12460 0.37 -1.0 2,710,302 - PS417_12460 0.38 +0.8 2,710,302 - PS417_12460 0.38 +2.0 2,710,302 - PS417_12460 0.38 +1.4 2,710,302 - PS417_12460 0.38 -0.6 2,710,326 + PS417_12460 0.47 -2.1 2,710,528 + -0.3 2,710,529 - +0.1 2,710,534 + +1.7 2,710,535 - +1.3 2,710,698 - +0.4 2,710,700 - -1.2 2,710,761 + +0.1 2,710,762 - -1.7 2,710,973 - +1.7 2,711,057 + PS417_12465 0.19 +1.3 2,711,110 - PS417_12465 0.23 -1.9 2,711,189 - PS417_12465 0.29 -1.1 2,711,544 - PS417_12465 0.56 -0.2 2,711,544 - PS417_12465 0.56 -0.4 2,711,693 - PS417_12465 0.67 -0.2 2,711,693 - PS417_12465 0.67 -1.6 2,711,753 + PS417_12465 0.71 +1.3 2,711,753 + PS417_12465 0.71 -0.3 2,711,753 + PS417_12465 0.71 -1.4 2,711,786 - PS417_12465 0.74 -2.2 2,711,927 + PS417_12465 0.84 -1.5 2,711,927 + PS417_12465 0.84 -0.4 2,711,927 + PS417_12465 0.84 +1.1 2,711,927 + PS417_12465 0.84 -0.3 2,711,928 - PS417_12465 0.85 -0.6 2,711,928 - PS417_12465 0.85 +0.6 2,711,928 - PS417_12465 0.85 -1.9 2,711,983 - PS417_12465 0.89 -0.1 2,712,249 - -1.0 2,712,249 - -1.2 2,712,365 + +1.3 2,712,465 + -0.3 2,712,493 + PS417_12470 0.19 -0.3 2,712,591 + PS417_12470 0.27 +2.0 2,712,599 + PS417_12470 0.27 -1.1 2,712,704 + PS417_12470 0.36 +1.4 2,712,704 + PS417_12470 0.36 +1.3 2,712,705 - PS417_12470 0.36 -1.5 2,712,705 - PS417_12470 0.36 -0.3 2,712,705 - PS417_12470 0.36 +1.0 2,712,859 - PS417_12470 0.47 -1.7 2,712,923 + PS417_12470 0.52 +0.2 2,712,987 - PS417_12470 0.57 +2.0 2,713,041 + PS417_12470 0.61 -0.1 2,713,056 + PS417_12470 0.63 +0.4 2,713,056 + PS417_12470 0.63 -0.1 2,713,057 - PS417_12470 0.63 -1.0 2,713,112 + PS417_12470 0.67 -0.7 2,713,112 + PS417_12470 0.67 +0.1
Or see this region's nucleotide sequence