Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12350 and PS417_12355 are separated by 55 nucleotides PS417_12355 and PS417_12360 are separated by 53 nucleotides PS417_12360 and PS417_12365 are separated by 114 nucleotides
PS417_12350: PS417_12350 - cobalamin biosynthesis protein CobN, at 2,684,319 to 2,688,080
_12350
PS417_12355: PS417_12355 - magnesium chelatase, at 2,688,136 to 2,689,134
_12355
PS417_12360: PS417_12360 - magnesium chelatase, at 2,689,188 to 2,689,790
_12360
PS417_12365: PS417_12365 - cobalt chelatase, at 2,689,905 to 2,690,852
_12365
Position (kb)
2688
2689
2690 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2687.141 kb on - strand, within PS417_12350 at 2687.404 kb on - strand, within PS417_12350 at 2687.490 kb on + strand, within PS417_12350 at 2687.616 kb on + strand, within PS417_12350 at 2687.616 kb on + strand, within PS417_12350 at 2687.616 kb on + strand, within PS417_12350 at 2687.617 kb on - strand, within PS417_12350 at 2687.617 kb on - strand, within PS417_12350 at 2687.617 kb on - strand, within PS417_12350 at 2687.805 kb on + strand at 2687.806 kb on - strand at 2688.064 kb on + strand at 2688.065 kb on - strand at 2688.065 kb on - strand at 2688.065 kb on - strand at 2688.065 kb on - strand at 2688.078 kb on + strand at 2688.442 kb on + strand, within PS417_12355 at 2688.443 kb on - strand, within PS417_12355 at 2688.939 kb on + strand, within PS417_12355 at 2688.940 kb on - strand, within PS417_12355 at 2689.294 kb on - strand, within PS417_12360 at 2689.775 kb on + strand at 2689.789 kb on - strand at 2689.890 kb on + strand at 2689.891 kb on - strand at 2689.891 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 2,687,141 - PS417_12350 0.75 -1.7 2,687,404 - PS417_12350 0.82 -4.4 2,687,490 + PS417_12350 0.84 -0.3 2,687,616 + PS417_12350 0.88 +1.0 2,687,616 + PS417_12350 0.88 +0.3 2,687,616 + PS417_12350 0.88 -1.7 2,687,617 - PS417_12350 0.88 -1.3 2,687,617 - PS417_12350 0.88 +1.0 2,687,617 - PS417_12350 0.88 -0.9 2,687,805 + -0.3 2,687,806 - +0.2 2,688,064 + -0.7 2,688,065 - -2.0 2,688,065 - -0.4 2,688,065 - -1.1 2,688,065 - +1.3 2,688,078 + +0.3 2,688,442 + PS417_12355 0.31 -1.5 2,688,443 - PS417_12355 0.31 -0.8 2,688,939 + PS417_12355 0.80 -1.3 2,688,940 - PS417_12355 0.80 -3.1 2,689,294 - PS417_12360 0.18 -2.5 2,689,775 + -1.7 2,689,789 - -0.2 2,689,890 + -0.1 2,689,891 - +0.0 2,689,891 - +1.3
Or see this region's nucleotide sequence