Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11670 and PS417_11675 overlap by 17 nucleotides PS417_11675 and PS417_11680 are separated by 140 nucleotides PS417_11680 and PS417_11690 are separated by 505 nucleotides
PS417_11670: PS417_11670 - multidrug transporter MATE, at 2,510,985 to 2,512,340
_11670
PS417_11675: PS417_11675 - TetR family transcriptional regulator, at 2,512,324 to 2,512,911
_11675
PS417_11680: PS417_11680 - MFS transporter, at 2,513,052 to 2,514,224
_11680
PS417_11690: PS417_11690 - peptide synthase, at 2,514,730 to 2,528,769
_11690
Position (kb)
2513
2514
2515 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2512.206 kb on + strand at 2512.206 kb on + strand at 2512.206 kb on + strand at 2512.914 kb on + strand at 2512.980 kb on - strand at 2513.105 kb on - strand at 2513.216 kb on - strand, within PS417_11680 at 2513.216 kb on - strand, within PS417_11680 at 2513.216 kb on - strand, within PS417_11680 at 2513.588 kb on - strand, within PS417_11680 at 2513.815 kb on - strand, within PS417_11680 at 2513.815 kb on - strand, within PS417_11680 at 2514.232 kb on - strand at 2514.232 kb on - strand at 2514.427 kb on - strand at 2514.429 kb on + strand at 2514.469 kb on + strand at 2514.470 kb on - strand at 2514.609 kb on + strand at 2514.610 kb on - strand at 2514.709 kb on + strand at 2514.943 kb on + strand at 2514.944 kb on - strand at 2514.944 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 2,512,206 + -1.0 2,512,206 + +0.2 2,512,206 + -0.8 2,512,914 + +0.4 2,512,980 - +0.6 2,513,105 - -1.3 2,513,216 - PS417_11680 0.14 +1.3 2,513,216 - PS417_11680 0.14 -1.5 2,513,216 - PS417_11680 0.14 +0.8 2,513,588 - PS417_11680 0.46 +0.6 2,513,815 - PS417_11680 0.65 -0.8 2,513,815 - PS417_11680 0.65 +2.2 2,514,232 - -0.2 2,514,232 - +2.1 2,514,427 - +0.7 2,514,429 + +0.0 2,514,469 + -1.0 2,514,470 - +1.4 2,514,609 + -0.7 2,514,610 - -0.5 2,514,709 + -0.4 2,514,943 + -0.7 2,514,944 - -1.8 2,514,944 - -2.9
Or see this region's nucleotide sequence