Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_10970 and PS417_10980 are separated by 423 nucleotides PS417_10980 and PS417_10985 are separated by 90 nucleotides PS417_10985 and PS417_10990 are separated by 174 nucleotides
PS417_10970: PS417_10970 - effector protein, at 2,350,268 to 2,351,158
_10970
PS417_10980: PS417_10980 - ArsR family transcriptional regulator, at 2,351,582 to 2,351,881
_10980
PS417_10985: PS417_10985 - NADH:flavin oxidoreductase, at 2,351,972 to 2,353,063
_10985
PS417_10990: PS417_10990 - TonB-dependent receptor, at 2,353,238 to 2,355,355
_10990
Position (kb)
2351
2352
2353
2354 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2351.031 kb on + strand, within PS417_10970 at 2351.031 kb on + strand, within PS417_10970 at 2351.120 kb on - strand at 2351.194 kb on - strand at 2351.332 kb on + strand at 2351.348 kb on - strand at 2351.870 kb on - strand at 2351.893 kb on + strand at 2351.893 kb on + strand at 2352.031 kb on + strand at 2352.050 kb on + strand at 2352.050 kb on + strand at 2352.051 kb on - strand at 2352.051 kb on - strand at 2352.051 kb on - strand at 2352.188 kb on - strand, within PS417_10985 at 2352.680 kb on + strand at 2352.978 kb on - strand at 2353.072 kb on - strand at 2353.171 kb on - strand at 2353.200 kb on + strand at 2353.365 kb on - strand at 2353.713 kb on - strand, within PS417_10990 at 2353.876 kb on + strand, within PS417_10990
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 2,351,031 + PS417_10970 0.86 -1.5 2,351,031 + PS417_10970 0.86 +0.1 2,351,120 - -0.2 2,351,194 - +0.1 2,351,332 + -0.7 2,351,348 - -1.7 2,351,870 - +0.3 2,351,893 + -0.7 2,351,893 + -0.4 2,352,031 + -0.1 2,352,050 + +2.3 2,352,050 + +2.3 2,352,051 - +1.0 2,352,051 - +1.0 2,352,051 - -1.8 2,352,188 - PS417_10985 0.20 -1.0 2,352,680 + -0.2 2,352,978 - -0.2 2,353,072 - +0.3 2,353,171 - +1.4 2,353,200 + +0.2 2,353,365 - +1.6 2,353,713 - PS417_10990 0.22 -2.1 2,353,876 + PS417_10990 0.30 +0.4
Or see this region's nucleotide sequence