Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_10935 and PS417_10940 overlap by 4 nucleotides PS417_10940 and PS417_10945 are separated by 101 nucleotides
PS417_10935: PS417_10935 - xylose transporter, at 2,338,953 to 2,340,509
_10935
PS417_10940: PS417_10940 - sugar ABC transporter substrate-binding protein, at 2,340,506 to 2,341,507
_10940
PS417_10945: PS417_10945 - Xylose isomerase (EC 5.3.1.5) (from data) , at 2,341,609 to 2,342,925
_10945
Position (kb)
2340
2341
2342 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2339.752 kb on + strand, within PS417_10935 at 2339.902 kb on + strand, within PS417_10935 at 2339.902 kb on + strand, within PS417_10935 at 2339.902 kb on + strand, within PS417_10935 at 2340.562 kb on + strand at 2340.628 kb on - strand, within PS417_10940 at 2340.639 kb on + strand, within PS417_10940 at 2340.640 kb on - strand, within PS417_10940 at 2340.640 kb on - strand, within PS417_10940 at 2340.640 kb on - strand, within PS417_10940 at 2340.715 kb on - strand, within PS417_10940 at 2341.059 kb on + strand, within PS417_10940 at 2341.064 kb on - strand, within PS417_10940 at 2341.134 kb on + strand, within PS417_10940 at 2341.135 kb on - strand, within PS417_10940 at 2341.171 kb on - strand, within PS417_10940 at 2341.254 kb on - strand at 2341.368 kb on + strand, within PS417_10940 at 2341.511 kb on - strand at 2341.524 kb on - strand at 2341.617 kb on - strand at 2341.844 kb on - strand, within PS417_10945 at 2341.922 kb on + strand, within PS417_10945 at 2342.159 kb on + strand at 2342.201 kb on + strand, within PS417_10945 at 2342.375 kb on + strand, within PS417_10945 at 2342.375 kb on + strand, within PS417_10945 at 2342.375 kb on + strand, within PS417_10945
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 2,339,752 + PS417_10935 0.51 +0.6 2,339,902 + PS417_10935 0.61 +1.3 2,339,902 + PS417_10935 0.61 +1.3 2,339,902 + PS417_10935 0.61 +0.3 2,340,562 + +0.6 2,340,628 - PS417_10940 0.12 -3.4 2,340,639 + PS417_10940 0.13 -0.3 2,340,640 - PS417_10940 0.13 +0.2 2,340,640 - PS417_10940 0.13 +0.3 2,340,640 - PS417_10940 0.13 -0.2 2,340,715 - PS417_10940 0.21 -2.5 2,341,059 + PS417_10940 0.55 -0.2 2,341,064 - PS417_10940 0.56 -0.6 2,341,134 + PS417_10940 0.63 -1.0 2,341,135 - PS417_10940 0.63 -0.7 2,341,171 - PS417_10940 0.66 -0.7 2,341,254 - +1.3 2,341,368 + PS417_10940 0.86 +0.1 2,341,511 - -1.2 2,341,524 - -2.2 2,341,617 - +0.5 2,341,844 - PS417_10945 0.18 +0.3 2,341,922 + PS417_10945 0.24 -1.2 2,342,159 + -1.8 2,342,201 + PS417_10945 0.45 -0.3 2,342,375 + PS417_10945 0.58 -0.7 2,342,375 + PS417_10945 0.58 +2.7 2,342,375 + PS417_10945 0.58 -0.4
Or see this region's nucleotide sequence