Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_10690 and PS417_10695 overlap by 11 nucleotides PS417_10695 and PS417_10700 are separated by 87 nucleotides PS417_10700 and PS417_10705 are separated by 19 nucleotides
PS417_10690: PS417_10690 - acyl-CoA synthetase, at 2,283,223 to 2,285,331
_10690
PS417_10695: PS417_10695 - decarboxylase, at 2,285,321 to 2,286,928
_10695
PS417_10700: PS417_10700 - NAD(P)H dehydrogenase, at 2,287,016 to 2,287,717
_10700
PS417_10705: PS417_10705 - phospho-2-dehydro-3-deoxyheptonate aldolase, at 2,287,737 to 2,288,816
_10705
Position (kb)
2285
2286
2287 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2284.359 kb on - strand, within PS417_10690 at 2284.717 kb on - strand, within PS417_10690 at 2284.717 kb on - strand at 2284.734 kb on - strand, within PS417_10690 at 2285.324 kb on + strand at 2285.324 kb on + strand at 2285.325 kb on - strand at 2285.325 kb on - strand at 2285.325 kb on - strand at 2285.325 kb on - strand at 2285.426 kb on + strand at 2285.426 kb on + strand at 2285.806 kb on + strand, within PS417_10695 at 2285.807 kb on - strand, within PS417_10695 at 2286.246 kb on - strand, within PS417_10695 at 2286.494 kb on + strand, within PS417_10695 at 2286.494 kb on + strand, within PS417_10695 at 2286.495 kb on - strand, within PS417_10695 at 2286.641 kb on + strand, within PS417_10695 at 2286.641 kb on + strand, within PS417_10695 at 2286.642 kb on - strand, within PS417_10695 at 2286.852 kb on + strand at 2287.196 kb on + strand, within PS417_10700 at 2287.203 kb on + strand, within PS417_10700 at 2287.203 kb on + strand, within PS417_10700 at 2287.203 kb on + strand, within PS417_10700 at 2287.203 kb on + strand, within PS417_10700 at 2287.203 kb on + strand, within PS417_10700 at 2287.204 kb on - strand, within PS417_10700 at 2287.205 kb on + strand, within PS417_10700 at 2287.206 kb on - strand, within PS417_10700 at 2287.256 kb on - strand, within PS417_10700 at 2287.400 kb on + strand, within PS417_10700 at 2287.465 kb on + strand, within PS417_10700 at 2287.465 kb on + strand, within PS417_10700 at 2287.505 kb on - strand, within PS417_10700 at 2287.538 kb on + strand, within PS417_10700 at 2287.586 kb on + strand, within PS417_10700 at 2287.587 kb on - strand, within PS417_10700 at 2287.703 kb on + strand at 2287.703 kb on + strand at 2287.703 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 2,284,359 - PS417_10690 0.54 +0.3 2,284,717 - PS417_10690 0.71 +0.6 2,284,717 - +0.3 2,284,734 - PS417_10690 0.72 -1.6 2,285,324 + +0.6 2,285,324 + +0.5 2,285,325 - -1.7 2,285,325 - -0.4 2,285,325 - -1.0 2,285,325 - -0.7 2,285,426 + -0.2 2,285,426 + -0.4 2,285,806 + PS417_10695 0.30 -1.8 2,285,807 - PS417_10695 0.30 -2.4 2,286,246 - PS417_10695 0.58 +1.3 2,286,494 + PS417_10695 0.73 -0.9 2,286,494 + PS417_10695 0.73 +0.3 2,286,495 - PS417_10695 0.73 +0.1 2,286,641 + PS417_10695 0.82 -1.2 2,286,641 + PS417_10695 0.82 -3.3 2,286,642 - PS417_10695 0.82 -1.0 2,286,852 + +0.5 2,287,196 + PS417_10700 0.26 +0.3 2,287,203 + PS417_10700 0.27 -0.4 2,287,203 + PS417_10700 0.27 +1.3 2,287,203 + PS417_10700 0.27 +1.0 2,287,203 + PS417_10700 0.27 +1.0 2,287,203 + PS417_10700 0.27 +0.5 2,287,204 - PS417_10700 0.27 -1.7 2,287,205 + PS417_10700 0.27 -1.5 2,287,206 - PS417_10700 0.27 +1.0 2,287,256 - PS417_10700 0.34 +1.0 2,287,400 + PS417_10700 0.55 -1.8 2,287,465 + PS417_10700 0.64 -1.5 2,287,465 + PS417_10700 0.64 -0.7 2,287,505 - PS417_10700 0.70 +1.3 2,287,538 + PS417_10700 0.74 +1.3 2,287,586 + PS417_10700 0.81 -1.8 2,287,587 - PS417_10700 0.81 -0.3 2,287,703 + +1.0 2,287,703 + -1.1 2,287,703 + -1.0
Or see this region's nucleotide sequence