Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01025 and PS417_01030 overlap by 4 nucleotides PS417_01030 and PS417_01035 are separated by 99 nucleotides PS417_01035 and PS417_01040 are separated by 121 nucleotides
PS417_01025: PS417_01025 - glutamine ABC transporter ATP-binding protein, at 223,171 to 223,947
_01025
PS417_01030: PS417_01030 - glutamine ABC transporter substrate-binding protein, at 223,944 to 224,798
_01030
PS417_01035: PS417_01035 - ATP-binding protein, at 224,898 to 226,058
_01035
PS417_01040: PS417_01040 - RND transporter, at 226,180 to 227,262
_01040
Position (kb) 224
225
226
227 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 223.944 kb on - strand at 224.134 kb on - strand, within PS417_01030 at 224.433 kb on - strand, within PS417_01030 at 224.724 kb on + strand at 224.724 kb on + strand at 224.725 kb on - strand at 224.725 kb on - strand at 224.838 kb on + strand at 224.838 kb on + strand at 224.838 kb on + strand at 224.839 kb on - strand at 224.839 kb on - strand at 224.887 kb on - strand at 224.899 kb on + strand at 224.900 kb on - strand at 224.988 kb on - strand at 225.184 kb on + strand, within PS417_01035 at 225.553 kb on + strand, within PS417_01035 at 225.553 kb on + strand, within PS417_01035 at 225.554 kb on - strand, within PS417_01035 at 225.554 kb on - strand, within PS417_01035 at 226.110 kb on + strand at 226.125 kb on + strand at 226.126 kb on - strand at 226.339 kb on - strand, within PS417_01040 at 226.542 kb on - strand, within PS417_01040 at 226.570 kb on + strand, within PS417_01040 at 226.570 kb on + strand, within PS417_01040 at 226.570 kb on + strand, within PS417_01040 at 226.571 kb on - strand, within PS417_01040 at 226.746 kb on - strand, within PS417_01040 at 226.811 kb on - strand, within PS417_01040 at 226.811 kb on - strand, within PS417_01040 at 226.811 kb on - strand, within PS417_01040 at 226.929 kb on + strand, within PS417_01040
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 223,944 - -1.9 224,134 - PS417_01030 0.22 -0.9 224,433 - PS417_01030 0.57 -0.7 224,724 + +0.3 224,724 + -2.1 224,725 - +0.0 224,725 - -1.7 224,838 + -1.0 224,838 + -0.0 224,838 + -2.2 224,839 - -0.2 224,839 - -0.1 224,887 - +1.8 224,899 + +0.4 224,900 - +1.6 224,988 - -1.0 225,184 + PS417_01035 0.25 -0.7 225,553 + PS417_01035 0.56 -1.1 225,553 + PS417_01035 0.56 -2.5 225,554 - PS417_01035 0.57 -0.7 225,554 - PS417_01035 0.57 -0.5 226,110 + -1.1 226,125 + -0.3 226,126 - +0.4 226,339 - PS417_01040 0.15 -0.2 226,542 - PS417_01040 0.33 -0.5 226,570 + PS417_01040 0.36 -3.0 226,570 + PS417_01040 0.36 +1.1 226,570 + PS417_01040 0.36 +0.5 226,571 - PS417_01040 0.36 +0.8 226,746 - PS417_01040 0.52 +0.4 226,811 - PS417_01040 0.58 +1.0 226,811 - PS417_01040 0.58 +2.1 226,811 - PS417_01040 0.58 -1.1 226,929 + PS417_01040 0.69 +1.8
Or see this region's nucleotide sequence