Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_09665 and PS417_09670 are separated by 90 nucleotides PS417_09670 and PS417_09675 overlap by 67 nucleotides PS417_09675 and PS417_09680 are separated by 74 nucleotides
PS417_09665: PS417_09665 - dimethylglycine dehydrogenase, at 2,096,198 to 2,096,878
_09665
PS417_09670: PS417_09670 - AraC family transcriptional regulator, at 2,096,969 to 2,097,973
_09670
PS417_09675: PS417_09675 - alpha/beta hydrolase, at 2,097,907 to 2,098,821
_09675
PS417_09680: PS417_09680 - hydrolase, at 2,098,896 to 2,099,369
_09680
Position (kb)
2096
2097
2098 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2096.015 kb on + strand at 2096.054 kb on + strand at 2096.145 kb on + strand at 2096.153 kb on + strand at 2096.154 kb on - strand at 2096.154 kb on - strand at 2096.154 kb on - strand at 2096.154 kb on - strand at 2096.521 kb on + strand, within PS417_09665 at 2096.709 kb on + strand, within PS417_09665 at 2096.710 kb on - strand, within PS417_09665 at 2096.710 kb on - strand, within PS417_09665 at 2096.710 kb on - strand, within PS417_09665 at 2096.917 kb on - strand at 2096.924 kb on + strand at 2096.925 kb on - strand at 2096.939 kb on - strand at 2097.190 kb on + strand, within PS417_09670 at 2097.978 kb on + strand at 2097.978 kb on + strand at 2097.978 kb on + strand at 2097.979 kb on - strand at 2098.185 kb on - strand, within PS417_09675 at 2098.244 kb on + strand, within PS417_09675 at 2098.244 kb on + strand, within PS417_09675 at 2098.244 kb on + strand, within PS417_09675 at 2098.244 kb on + strand, within PS417_09675 at 2098.361 kb on + strand, within PS417_09675 at 2098.427 kb on + strand, within PS417_09675 at 2098.427 kb on + strand, within PS417_09675 at 2098.483 kb on - strand, within PS417_09675 at 2098.648 kb on + strand, within PS417_09675 at 2098.649 kb on - strand, within PS417_09675 at 2098.695 kb on - strand, within PS417_09675 at 2098.766 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 2,096,015 + +1.1 2,096,054 + -0.5 2,096,145 + -0.6 2,096,153 + +1.5 2,096,154 - -0.2 2,096,154 - -0.6 2,096,154 - +2.1 2,096,154 - -0.1 2,096,521 + PS417_09665 0.47 -0.2 2,096,709 + PS417_09665 0.75 +1.8 2,096,710 - PS417_09665 0.75 -0.0 2,096,710 - PS417_09665 0.75 +0.1 2,096,710 - PS417_09665 0.75 -0.2 2,096,917 - +3.3 2,096,924 + +0.1 2,096,925 - +0.6 2,096,939 - -1.2 2,097,190 + PS417_09670 0.22 -0.4 2,097,978 + +0.1 2,097,978 + -1.4 2,097,978 + +1.4 2,097,979 - +0.4 2,098,185 - PS417_09675 0.30 +0.5 2,098,244 + PS417_09675 0.37 -1.6 2,098,244 + PS417_09675 0.37 +0.2 2,098,244 + PS417_09675 0.37 -0.2 2,098,244 + PS417_09675 0.37 +0.1 2,098,361 + PS417_09675 0.50 +0.0 2,098,427 + PS417_09675 0.57 +0.8 2,098,427 + PS417_09675 0.57 +1.4 2,098,483 - PS417_09675 0.63 +0.5 2,098,648 + PS417_09675 0.81 -1.8 2,098,649 - PS417_09675 0.81 -1.0 2,098,695 - PS417_09675 0.86 -0.5 2,098,766 + -1.0
Or see this region's nucleotide sequence