Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00085 and PS417_00090 overlap by 4 nucleotides PS417_00090 and PS417_00095 are separated by 69 nucleotides PS417_00095 and PS417_00100 are separated by 135 nucleotides
PS417_00085: PS417_00085 - 16S rRNA methyltransferase, at 18,722 to 20,032
_00085
PS417_00090: PS417_00090 - methionyl-tRNA formyltransferase, at 20,029 to 20,982
_00090
PS417_00095: PS417_00095 - peptide deformylase, at 21,052 to 21,558
_00095
PS417_00100: PS417_00100 - peptidoglycan-binding protein, at 21,694 to 22,470
_00100
Position (kb) 20
21 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 19.777 kb on - strand, within PS417_00085 at 19.777 kb on - strand, within PS417_00085 at 20.997 kb on - strand at 21.565 kb on - strand at 21.565 kb on - strand at 21.565 kb on - strand at 21.565 kb on - strand at 21.585 kb on - strand at 21.675 kb on - strand at 21.678 kb on - strand at 21.678 kb on - strand at 21.678 kb on - strand at 21.720 kb on - strand at 21.720 kb on - strand at 21.856 kb on + strand, within PS417_00100 at 21.856 kb on + strand, within PS417_00100 at 21.857 kb on - strand, within PS417_00100 at 21.857 kb on - strand, within PS417_00100 at 21.857 kb on - strand, within PS417_00100 at 21.953 kb on + strand, within PS417_00100 at 21.973 kb on + strand, within PS417_00100
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 19,777 - PS417_00085 0.80 -1.8 19,777 - PS417_00085 0.80 -0.7 20,997 - +0.2 21,565 - +0.9 21,565 - +0.0 21,565 - +0.8 21,565 - -1.1 21,585 - +1.5 21,675 - +0.3 21,678 - -0.1 21,678 - -0.5 21,678 - -0.6 21,720 - -0.1 21,720 - +1.5 21,856 + PS417_00100 0.21 -3.2 21,856 + PS417_00100 0.21 -1.5 21,857 - PS417_00100 0.21 -1.9 21,857 - PS417_00100 0.21 +0.6 21,857 - PS417_00100 0.21 -1.3 21,953 + PS417_00100 0.33 -0.1 21,973 + PS417_00100 0.36 -0.3
Or see this region's nucleotide sequence