Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_05005 and PS417_05010 are separated by 1 nucleotides PS417_05010 and PS417_05015 are separated by 120 nucleotides PS417_05015 and PS417_05020 are separated by 3 nucleotides
PS417_05005: PS417_05005 - penicillin-binding protein, at 1,113,121 to 1,114,566
_05005
PS417_05010: PS417_05010 - IclR family transcriptional regulator, at 1,114,568 to 1,115,347
_05010
PS417_05015: PS417_05015 - homogentisate 1,2-dioxygenase, at 1,115,468 to 1,116,751
_05015
PS417_05020: PS417_05020 - fumarylacetoacetase, at 1,116,755 to 1,118,041
_05020
Position (kb)
1115
1116
1117 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1114.492 kb on + strand at 1114.492 kb on + strand at 1114.492 kb on + strand at 1114.492 kb on + strand at 1114.493 kb on - strand at 1114.564 kb on + strand at 1114.565 kb on - strand at 1114.578 kb on + strand at 1114.803 kb on + strand, within PS417_05010 at 1114.803 kb on + strand, within PS417_05010 at 1114.804 kb on - strand, within PS417_05010 at 1115.197 kb on - strand at 1115.290 kb on - strand at 1115.385 kb on - strand at 1115.386 kb on + strand at 1115.386 kb on + strand at 1115.386 kb on + strand at 1115.387 kb on - strand at 1115.387 kb on - strand at 1115.413 kb on + strand at 1115.481 kb on - strand at 1115.481 kb on - strand at 1115.793 kb on + strand, within PS417_05015 at 1115.794 kb on - strand, within PS417_05015 at 1115.843 kb on + strand, within PS417_05015 at 1115.843 kb on + strand, within PS417_05015 at 1115.933 kb on - strand, within PS417_05015 at 1115.933 kb on - strand, within PS417_05015 at 1116.349 kb on + strand at 1116.461 kb on + strand, within PS417_05015 at 1116.462 kb on - strand, within PS417_05015 at 1116.538 kb on + strand, within PS417_05015 at 1116.648 kb on + strand at 1116.698 kb on + strand at 1116.767 kb on - strand at 1117.217 kb on + strand, within PS417_05020
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 1,114,492 + +2.3 1,114,492 + -1.2 1,114,492 + +0.1 1,114,492 + -0.3 1,114,493 - -0.8 1,114,564 + +1.5 1,114,565 - -0.3 1,114,578 + +0.4 1,114,803 + PS417_05010 0.30 -0.8 1,114,803 + PS417_05010 0.30 -1.2 1,114,804 - PS417_05010 0.30 -0.1 1,115,197 - +1.5 1,115,290 - +0.5 1,115,385 - +0.5 1,115,386 + -0.7 1,115,386 + -0.3 1,115,386 + +0.4 1,115,387 - -1.3 1,115,387 - +0.0 1,115,413 + -0.7 1,115,481 - +2.3 1,115,481 - -0.2 1,115,793 + PS417_05015 0.25 +0.2 1,115,794 - PS417_05015 0.25 +1.1 1,115,843 + PS417_05015 0.29 +0.2 1,115,843 + PS417_05015 0.29 +0.6 1,115,933 - PS417_05015 0.36 +3.5 1,115,933 - PS417_05015 0.36 -0.8 1,116,349 + -1.3 1,116,461 + PS417_05015 0.77 +2.7 1,116,462 - PS417_05015 0.77 -0.4 1,116,538 + PS417_05015 0.83 +0.8 1,116,648 + -0.8 1,116,698 + +2.1 1,116,767 - -1.1 1,117,217 + PS417_05020 0.36 +0.5
Or see this region's nucleotide sequence