Strain Fitness in Pseudomonas simiae WCS417 around PS417_05015

Experiment: JP1 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_05005 and PS417_05010 are separated by 1 nucleotidesPS417_05010 and PS417_05015 are separated by 120 nucleotidesPS417_05015 and PS417_05020 are separated by 3 nucleotides PS417_05005: PS417_05005 - penicillin-binding protein, at 1,113,121 to 1,114,566 _05005 PS417_05010: PS417_05010 - IclR family transcriptional regulator, at 1,114,568 to 1,115,347 _05010 PS417_05015: PS417_05015 - homogentisate 1,2-dioxygenase, at 1,115,468 to 1,116,751 _05015 PS417_05020: PS417_05020 - fumarylacetoacetase, at 1,116,755 to 1,118,041 _05020 Position (kb) 1115 1116 1117Strain fitness (log2 ratio) -1 0 1 2 3at 1114.492 kb on + strandat 1114.492 kb on + strandat 1114.492 kb on + strandat 1114.492 kb on + strandat 1114.493 kb on - strandat 1114.564 kb on + strandat 1114.565 kb on - strandat 1114.578 kb on + strandat 1114.803 kb on + strand, within PS417_05010at 1114.803 kb on + strand, within PS417_05010at 1114.804 kb on - strand, within PS417_05010at 1115.197 kb on - strandat 1115.290 kb on - strandat 1115.385 kb on - strandat 1115.386 kb on + strandat 1115.386 kb on + strandat 1115.386 kb on + strandat 1115.387 kb on - strandat 1115.387 kb on - strandat 1115.413 kb on + strandat 1115.481 kb on - strandat 1115.481 kb on - strandat 1115.793 kb on + strand, within PS417_05015at 1115.794 kb on - strand, within PS417_05015at 1115.843 kb on + strand, within PS417_05015at 1115.843 kb on + strand, within PS417_05015at 1115.933 kb on - strand, within PS417_05015at 1115.933 kb on - strand, within PS417_05015at 1116.349 kb on + strandat 1116.461 kb on + strand, within PS417_05015at 1116.462 kb on - strand, within PS417_05015at 1116.538 kb on + strand, within PS417_05015at 1116.648 kb on + strandat 1116.698 kb on + strandat 1116.767 kb on - strandat 1117.217 kb on + strand, within PS417_05020

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Per-strain Table

Position Strand Gene LocusTag Fraction JP1 MOI 1
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1,114,492 + +2.3
1,114,492 + -1.2
1,114,492 + +0.1
1,114,492 + -0.3
1,114,493 - -0.8
1,114,564 + +1.5
1,114,565 - -0.3
1,114,578 + +0.4
1,114,803 + PS417_05010 0.30 -0.8
1,114,803 + PS417_05010 0.30 -1.2
1,114,804 - PS417_05010 0.30 -0.1
1,115,197 - +1.5
1,115,290 - +0.5
1,115,385 - +0.5
1,115,386 + -0.7
1,115,386 + -0.3
1,115,386 + +0.4
1,115,387 - -1.3
1,115,387 - +0.0
1,115,413 + -0.7
1,115,481 - +2.3
1,115,481 - -0.2
1,115,793 + PS417_05015 0.25 +0.2
1,115,794 - PS417_05015 0.25 +1.1
1,115,843 + PS417_05015 0.29 +0.2
1,115,843 + PS417_05015 0.29 +0.6
1,115,933 - PS417_05015 0.36 +3.5
1,115,933 - PS417_05015 0.36 -0.8
1,116,349 + -1.3
1,116,461 + PS417_05015 0.77 +2.7
1,116,462 - PS417_05015 0.77 -0.4
1,116,538 + PS417_05015 0.83 +0.8
1,116,648 + -0.8
1,116,698 + +2.1
1,116,767 - -1.1
1,117,217 + PS417_05020 0.36 +0.5

Or see this region's nucleotide sequence