Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_03775 and PS417_03780 overlap by 47 nucleotides PS417_03780 and PS417_03785 are separated by 192 nucleotides PS417_03785 and PS417_03790 overlap by 4 nucleotides
PS417_03775: PS417_03775 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, at 855,075 to 856,022
_03775
PS417_03780: PS417_03780 - general secretion pathway protein GspH, at 855,976 to 856,557
_03780
PS417_03785: PS417_03785 - pilus assembly protein PilV, at 856,750 to 857,235
_03785
PS417_03790: PS417_03790 - pilus assembly protein PilW, at 857,232 to 857,939
_03790
Position (kb)
855
856
857 Strain fitness (log2 ratio)
-1
0
1
2 at 855.008 kb on + strand at 855.024 kb on + strand at 855.024 kb on + strand at 856.025 kb on - strand at 856.144 kb on + strand, within PS417_03780 at 856.144 kb on + strand, within PS417_03780 at 856.145 kb on - strand, within PS417_03780 at 856.557 kb on + strand at 856.558 kb on - strand at 856.558 kb on - strand at 856.594 kb on + strand at 856.594 kb on + strand at 856.594 kb on + strand at 856.595 kb on - strand at 856.595 kb on - strand at 856.595 kb on - strand at 856.597 kb on - strand at 856.641 kb on + strand at 856.642 kb on - strand at 856.685 kb on + strand at 856.895 kb on - strand, within PS417_03785 at 856.939 kb on + strand, within PS417_03785 at 857.327 kb on + strand, within PS417_03790
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 855,008 + +0.1 855,024 + +1.5 855,024 + -0.6 856,025 - -0.5 856,144 + PS417_03780 0.29 -0.9 856,144 + PS417_03780 0.29 -0.9 856,145 - PS417_03780 0.29 +0.6 856,557 + -0.3 856,558 - -0.2 856,558 - +0.6 856,594 + +2.3 856,594 + +1.1 856,594 + -0.9 856,595 - +0.5 856,595 - -0.9 856,595 - -0.5 856,597 - +0.9 856,641 + -0.9 856,642 - +1.9 856,685 + -0.2 856,895 - PS417_03785 0.30 -1.4 856,939 + PS417_03785 0.39 -1.3 857,327 + PS417_03790 0.13 -0.3
Or see this region's nucleotide sequence