Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_25885 and PS417_25890 are separated by 16 nucleotides PS417_25890 and PS417_25895 are separated by 45 nucleotides PS417_25895 and PS417_25900 are separated by 112 nucleotides PS417_25900 and PS417_25905 are separated by 40 nucleotides PS417_25905 and PS417_25910 are separated by 167 nucleotides
PS417_25885: PS417_25885 - diguanylate cyclase, at 5,590,243 to 5,593,986
_25885
PS417_25890: PS417_25890 - tRNA-Met, at 5,594,003 to 5,594,079
_25890
PS417_25895: PS417_25895 - glyoxalase, at 5,594,125 to 5,594,550
_25895
PS417_25900: PS417_25900 - LysR family transcriptional regulator, at 5,594,663 to 5,595,532
_25900
PS417_25905: PS417_25905 - AsnC family transcriptional regulator, at 5,595,573 to 5,596,019
_25905
PS417_25910: PS417_25910 - amine oxidase, at 5,596,187 to 5,597,869
_25910
Position (kb)
5594
5595
5596 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 5593.678 kb on + strand at 5593.679 kb on - strand at 5593.936 kb on - strand at 5593.936 kb on - strand at 5593.982 kb on + strand at 5594.487 kb on - strand, within PS417_25895 at 5594.514 kb on - strand at 5594.514 kb on - strand at 5594.573 kb on + strand at 5594.573 kb on + strand at 5594.573 kb on + strand at 5594.573 kb on + strand at 5594.574 kb on - strand at 5594.574 kb on - strand at 5594.574 kb on - strand at 5594.588 kb on + strand at 5595.129 kb on + strand, within PS417_25900 at 5595.222 kb on + strand, within PS417_25900 at 5595.222 kb on + strand, within PS417_25900 at 5595.222 kb on + strand, within PS417_25900 at 5595.223 kb on - strand, within PS417_25900 at 5595.223 kb on - strand, within PS417_25900 at 5595.223 kb on - strand, within PS417_25900 at 5595.223 kb on - strand, within PS417_25900 at 5595.292 kb on - strand, within PS417_25900 at 5595.292 kb on - strand, within PS417_25900 at 5595.530 kb on + strand at 5595.530 kb on + strand at 5595.777 kb on + strand, within PS417_25905 at 5595.778 kb on - strand, within PS417_25905 at 5595.882 kb on + strand, within PS417_25905 at 5595.883 kb on - strand, within PS417_25905 at 5596.120 kb on + strand at 5596.121 kb on - strand at 5596.121 kb on - strand at 5596.122 kb on + strand at 5596.123 kb on - strand at 5596.407 kb on - strand, within PS417_25910 at 5596.407 kb on - strand, within PS417_25910 at 5596.517 kb on + strand, within PS417_25910 at 5596.518 kb on - strand, within PS417_25910 at 5596.518 kb on - strand, within PS417_25910 at 5596.523 kb on + strand, within PS417_25910
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 5,593,678 + -0.7 5,593,679 - -1.3 5,593,936 - +0.5 5,593,936 - +2.0 5,593,982 + -1.6 5,594,487 - PS417_25895 0.85 -0.5 5,594,514 - -0.2 5,594,514 - -4.4 5,594,573 + +0.5 5,594,573 + +1.1 5,594,573 + +1.5 5,594,573 + -0.5 5,594,574 - -0.1 5,594,574 - -0.5 5,594,574 - +3.1 5,594,588 + -0.8 5,595,129 + PS417_25900 0.54 +0.9 5,595,222 + PS417_25900 0.64 +1.3 5,595,222 + PS417_25900 0.64 +2.5 5,595,222 + PS417_25900 0.64 -1.0 5,595,223 - PS417_25900 0.64 +0.8 5,595,223 - PS417_25900 0.64 +0.4 5,595,223 - PS417_25900 0.64 -0.2 5,595,223 - PS417_25900 0.64 +0.3 5,595,292 - PS417_25900 0.72 -3.4 5,595,292 - PS417_25900 0.72 -0.4 5,595,530 + +0.8 5,595,530 + -0.7 5,595,777 + PS417_25905 0.46 +0.6 5,595,778 - PS417_25905 0.46 -1.2 5,595,882 + PS417_25905 0.69 -0.1 5,595,883 - PS417_25905 0.69 +0.6 5,596,120 + -0.1 5,596,121 - -0.2 5,596,121 - +0.8 5,596,122 + -0.7 5,596,123 - -0.8 5,596,407 - PS417_25910 0.13 +1.1 5,596,407 - PS417_25910 0.13 -0.8 5,596,517 + PS417_25910 0.20 +0.3 5,596,518 - PS417_25910 0.20 -3.3 5,596,518 - PS417_25910 0.20 -0.9 5,596,523 + PS417_25910 0.20 -0.5
Or see this region's nucleotide sequence