Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02465 and PS417_02470 overlap by 13 nucleotides PS417_02470 and PS417_02475 are separated by 15 nucleotides PS417_02475 and PS417_02480 overlap by 1 nucleotides
PS417_02465: PS417_02465 - carbohydrate kinase, at 563,744 to 564,604
_02465
PS417_02470: PS417_02470 - ATPase, at 564,592 to 565,062
_02470
PS417_02475: PS417_02475 - N-acetylmuramoyl-L-alanine amidase, at 565,078 to 566,499
_02475
PS417_02480: PS417_02480 - DNA mismatch repair protein, at 566,499 to 568,415
_02480
Position (kb) 565
566
567 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 564.275 kb on - strand, within PS417_02465 at 564.465 kb on - strand, within PS417_02465 at 564.555 kb on + strand at 564.602 kb on + strand at 566.296 kb on + strand, within PS417_02475 at 566.296 kb on + strand, within PS417_02475 at 566.296 kb on + strand at 566.297 kb on - strand, within PS417_02475 at 566.297 kb on - strand, within PS417_02475 at 566.297 kb on - strand, within PS417_02475 at 566.297 kb on - strand, within PS417_02475 at 566.409 kb on + strand at 567.285 kb on + strand, within PS417_02480 at 567.285 kb on + strand, within PS417_02480
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 564,275 - PS417_02465 0.62 -1.1 564,465 - PS417_02465 0.84 -1.1 564,555 + +1.2 564,602 + +0.7 566,296 + PS417_02475 0.86 -0.1 566,296 + PS417_02475 0.86 -3.0 566,296 + +2.5 566,297 - PS417_02475 0.86 -2.4 566,297 - PS417_02475 0.86 +1.5 566,297 - PS417_02475 0.86 -1.1 566,297 - PS417_02475 0.86 -2.5 566,409 + +3.0 567,285 + PS417_02480 0.41 -0.4 567,285 + PS417_02480 0.41 +1.8
Or see this region's nucleotide sequence