Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_21215 and PS417_21220 are separated by 16 nucleotides PS417_21220 and PS417_21230 are separated by 646 nucleotides
PS417_21215: PS417_21215 - 3-hydroxyacyl-CoA dehydrogenase, at 4,612,932 to 4,615,079
_21215
PS417_21220: PS417_21220 - acetyl-CoA acetyltransferase, at 4,615,096 to 4,616,301
_21220
PS417_21230: PS417_21230 - cytochrome C, at 4,616,948 to 4,617,751
_21230
Position (kb)
4615
4616
4617 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 4614.139 kb on + strand, within PS417_21215 at 4614.140 kb on - strand, within PS417_21215 at 4614.351 kb on - strand, within PS417_21215 at 4614.526 kb on - strand at 4614.706 kb on + strand, within PS417_21215 at 4614.915 kb on + strand at 4615.067 kb on + strand at 4615.067 kb on + strand at 4615.067 kb on + strand at 4615.583 kb on + strand, within PS417_21220 at 4615.583 kb on + strand, within PS417_21220 at 4615.707 kb on + strand, within PS417_21220 at 4615.707 kb on + strand, within PS417_21220 at 4615.707 kb on + strand, within PS417_21220 at 4615.881 kb on + strand, within PS417_21220 at 4615.881 kb on + strand at 4615.882 kb on - strand, within PS417_21220 at 4616.340 kb on - strand at 4616.417 kb on + strand at 4616.417 kb on + strand at 4616.423 kb on - strand at 4616.423 kb on - strand at 4616.558 kb on - strand at 4616.558 kb on - strand at 4616.711 kb on + strand at 4616.727 kb on + strand at 4616.728 kb on - strand at 4616.728 kb on - strand at 4616.728 kb on - strand at 4616.728 kb on - strand at 4616.800 kb on + strand at 4616.801 kb on - strand at 4616.801 kb on - strand at 4617.118 kb on + strand, within PS417_21230 at 4617.119 kb on - strand, within PS417_21230 at 4617.119 kb on - strand, within PS417_21230
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 4,614,139 + PS417_21215 0.56 -1.4 4,614,140 - PS417_21215 0.56 -2.0 4,614,351 - PS417_21215 0.66 +0.7 4,614,526 - -0.2 4,614,706 + PS417_21215 0.83 +1.4 4,614,915 + +1.5 4,615,067 + -1.4 4,615,067 + +0.1 4,615,067 + -2.0 4,615,583 + PS417_21220 0.40 -3.6 4,615,583 + PS417_21220 0.40 -0.9 4,615,707 + PS417_21220 0.51 -1.2 4,615,707 + PS417_21220 0.51 -0.9 4,615,707 + PS417_21220 0.51 -0.7 4,615,881 + PS417_21220 0.65 -1.1 4,615,881 + +1.0 4,615,882 - PS417_21220 0.65 -0.1 4,616,340 - +0.4 4,616,417 + +1.9 4,616,417 + -0.2 4,616,423 - +0.4 4,616,423 - +1.5 4,616,558 - +4.0 4,616,558 - +0.8 4,616,711 + +0.3 4,616,727 + +1.4 4,616,728 - -0.1 4,616,728 - -0.2 4,616,728 - +1.4 4,616,728 - +1.8 4,616,800 + -2.5 4,616,801 - +0.4 4,616,801 - +0.4 4,617,118 + PS417_21230 0.21 +0.6 4,617,119 - PS417_21230 0.21 +0.7 4,617,119 - PS417_21230 0.21 +0.5
Or see this region's nucleotide sequence