Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01985 and PS417_01990 are separated by 10 nucleotides PS417_01990 and PS417_01995 are separated by 98 nucleotides PS417_01995 and PS417_02000 overlap by 21 nucleotides
PS417_01985: PS417_01985 - 3-dehydroquinate synthase, at 440,397 to 441,497
_01985
PS417_01990: PS417_01990 - cell division protein, at 441,508 to 443,079
_01990
PS417_01995: PS417_01995 - hypothetical protein, at 443,178 to 443,453
_01995
PS417_02000: PS417_02000 - glutamate synthase, at 443,433 to 447,878
_02000
Position (kb) 441
442
443
444 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 441.489 kb on - strand at 441.548 kb on - strand at 441.861 kb on - strand, within PS417_01990 at 441.861 kb on - strand, within PS417_01990 at 441.889 kb on + strand, within PS417_01990 at 441.890 kb on - strand, within PS417_01990 at 441.983 kb on + strand, within PS417_01990 at 441.984 kb on - strand, within PS417_01990 at 441.984 kb on - strand, within PS417_01990 at 441.984 kb on - strand, within PS417_01990 at 442.073 kb on - strand, within PS417_01990 at 442.287 kb on + strand, within PS417_01990 at 442.298 kb on + strand, within PS417_01990 at 442.901 kb on - strand, within PS417_01990 at 442.951 kb on + strand at 443.149 kb on + strand at 443.149 kb on + strand at 443.149 kb on + strand at 443.236 kb on + strand, within PS417_01995 at 443.255 kb on + strand, within PS417_01995 at 443.317 kb on - strand, within PS417_01995 at 443.317 kb on - strand, within PS417_01995 at 443.520 kb on - strand at 443.686 kb on - strand at 443.916 kb on - strand, within PS417_02000 at 443.964 kb on + strand, within PS417_02000 at 443.997 kb on + strand, within PS417_02000 at 443.998 kb on - strand, within PS417_02000 at 443.998 kb on - strand, within PS417_02000 at 443.998 kb on - strand, within PS417_02000 at 443.999 kb on + strand, within PS417_02000 at 444.037 kb on - strand, within PS417_02000
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 441,489 - +0.1 441,548 - +1.3 441,861 - PS417_01990 0.22 +0.4 441,861 - PS417_01990 0.22 +2.2 441,889 + PS417_01990 0.24 +0.9 441,890 - PS417_01990 0.24 -3.7 441,983 + PS417_01990 0.30 -0.2 441,984 - PS417_01990 0.30 +0.5 441,984 - PS417_01990 0.30 +1.1 441,984 - PS417_01990 0.30 +2.2 442,073 - PS417_01990 0.36 +0.1 442,287 + PS417_01990 0.50 +3.8 442,298 + PS417_01990 0.50 +0.7 442,901 - PS417_01990 0.89 +1.4 442,951 + +1.0 443,149 + -0.0 443,149 + -0.4 443,149 + +0.0 443,236 + PS417_01995 0.21 -3.3 443,255 + PS417_01995 0.28 -0.6 443,317 - PS417_01995 0.50 +1.8 443,317 - PS417_01995 0.50 +0.9 443,520 - -0.3 443,686 - -1.0 443,916 - PS417_02000 0.11 +0.7 443,964 + PS417_02000 0.12 +1.8 443,997 + PS417_02000 0.13 -1.3 443,998 - PS417_02000 0.13 -2.8 443,998 - PS417_02000 0.13 +1.7 443,998 - PS417_02000 0.13 -0.8 443,999 + PS417_02000 0.13 +0.1 444,037 - PS417_02000 0.14 +0.8
Or see this region's nucleotide sequence