Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01975 and PS417_01980 are separated by 4 nucleotides PS417_01980 and PS417_01985 are separated by 125 nucleotides PS417_01985 and PS417_01990 are separated by 10 nucleotides
PS417_01975: PS417_01975 - pilus assembly protein PilQ, at 438,423 to 439,748
_01975
PS417_01980: PS417_01980 - shikimate kinase, at 439,753 to 440,271
_01980
PS417_01985: PS417_01985 - 3-dehydroquinate synthase, at 440,397 to 441,497
_01985
PS417_01990: PS417_01990 - cell division protein, at 441,508 to 443,079
_01990
Position (kb) 440
441
442 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 439.482 kb on + strand, within PS417_01975 at 439.483 kb on - strand, within PS417_01975 at 439.540 kb on + strand, within PS417_01975 at 439.606 kb on + strand, within PS417_01975 at 439.611 kb on - strand, within PS417_01975 at 439.611 kb on - strand, within PS417_01975 at 439.611 kb on - strand, within PS417_01975 at 439.637 kb on + strand at 439.715 kb on + strand at 441.489 kb on - strand at 441.548 kb on - strand at 441.861 kb on - strand, within PS417_01990 at 441.861 kb on - strand, within PS417_01990 at 441.889 kb on + strand, within PS417_01990 at 441.890 kb on - strand, within PS417_01990 at 441.983 kb on + strand, within PS417_01990 at 441.984 kb on - strand, within PS417_01990 at 441.984 kb on - strand, within PS417_01990 at 441.984 kb on - strand, within PS417_01990 at 442.073 kb on - strand, within PS417_01990 at 442.287 kb on + strand, within PS417_01990 at 442.298 kb on + strand, within PS417_01990
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 439,482 + PS417_01975 0.80 +2.7 439,483 - PS417_01975 0.80 -0.0 439,540 + PS417_01975 0.84 -0.0 439,606 + PS417_01975 0.89 -2.5 439,611 - PS417_01975 0.90 -0.5 439,611 - PS417_01975 0.90 +0.5 439,611 - PS417_01975 0.90 -0.5 439,637 + -1.8 439,715 + -2.4 441,489 - +0.1 441,548 - +1.3 441,861 - PS417_01990 0.22 +0.4 441,861 - PS417_01990 0.22 +2.2 441,889 + PS417_01990 0.24 +0.9 441,890 - PS417_01990 0.24 -3.7 441,983 + PS417_01990 0.30 -0.2 441,984 - PS417_01990 0.30 +0.5 441,984 - PS417_01990 0.30 +1.1 441,984 - PS417_01990 0.30 +2.2 442,073 - PS417_01990 0.36 +0.1 442,287 + PS417_01990 0.50 +3.8 442,298 + PS417_01990 0.50 +0.7
Or see this region's nucleotide sequence