Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_19445 and PS417_19450 are separated by 31 nucleotides PS417_19450 and PS417_19455 are separated by 30 nucleotides
PS417_19445: PS417_19445 - membrane protein, at 4,217,078 to 4,217,863
_19445
PS417_19450: PS417_19450 - Patatin, at 4,217,895 to 4,219,028
_19450
PS417_19455: PS417_19455 - lipid A biosynthesis lauroyl acyltransferase, at 4,219,059 to 4,219,994
_19455
Position (kb) 4217
4218
4219
4220 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 4216.966 kb on + strand at 4216.975 kb on + strand at 4216.975 kb on + strand at 4216.976 kb on - strand at 4217.039 kb on - strand at 4217.061 kb on - strand at 4217.086 kb on + strand at 4217.087 kb on - strand at 4217.180 kb on + strand, within PS417_19445 at 4217.180 kb on + strand, within PS417_19445 at 4217.181 kb on - strand, within PS417_19445 at 4217.181 kb on - strand, within PS417_19445 at 4217.181 kb on - strand, within PS417_19445 at 4217.181 kb on - strand, within PS417_19445 at 4217.333 kb on + strand, within PS417_19445 at 4217.333 kb on + strand, within PS417_19445 at 4217.334 kb on - strand, within PS417_19445 at 4217.336 kb on + strand, within PS417_19445 at 4217.337 kb on - strand, within PS417_19445 at 4217.429 kb on + strand, within PS417_19445 at 4217.429 kb on + strand, within PS417_19445 at 4217.429 kb on + strand, within PS417_19445 at 4217.429 kb on + strand, within PS417_19445 at 4217.430 kb on - strand, within PS417_19445 at 4217.460 kb on - strand, within PS417_19445 at 4217.505 kb on - strand, within PS417_19445 at 4217.523 kb on - strand, within PS417_19445 at 4217.676 kb on - strand, within PS417_19445 at 4217.711 kb on + strand, within PS417_19445 at 4217.711 kb on + strand, within PS417_19445 at 4217.753 kb on + strand, within PS417_19445 at 4217.753 kb on + strand at 4217.753 kb on + strand, within PS417_19445 at 4217.753 kb on + strand, within PS417_19445 at 4217.753 kb on + strand, within PS417_19445 at 4217.754 kb on - strand, within PS417_19445 at 4217.754 kb on - strand, within PS417_19445 at 4217.769 kb on - strand, within PS417_19445 at 4217.821 kb on + strand at 4217.853 kb on - strand at 4217.853 kb on - strand at 4218.007 kb on + strand at 4218.242 kb on + strand, within PS417_19450 at 4218.242 kb on + strand, within PS417_19450 at 4218.242 kb on + strand, within PS417_19450 at 4218.243 kb on - strand, within PS417_19450 at 4218.284 kb on - strand, within PS417_19450 at 4218.284 kb on - strand, within PS417_19450 at 4218.284 kb on - strand, within PS417_19450 at 4218.825 kb on + strand, within PS417_19450 at 4218.825 kb on + strand, within PS417_19450 at 4218.825 kb on + strand, within PS417_19450
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 4,216,966 + -0.8 4,216,975 + -2.3 4,216,975 + +0.4 4,216,976 - -3.3 4,217,039 - -0.2 4,217,061 - -0.9 4,217,086 + +1.7 4,217,087 - -0.6 4,217,180 + PS417_19445 0.13 +0.2 4,217,180 + PS417_19445 0.13 +0.6 4,217,181 - PS417_19445 0.13 +0.1 4,217,181 - PS417_19445 0.13 +1.1 4,217,181 - PS417_19445 0.13 -2.9 4,217,181 - PS417_19445 0.13 +0.0 4,217,333 + PS417_19445 0.32 +0.4 4,217,333 + PS417_19445 0.32 +0.9 4,217,334 - PS417_19445 0.33 -2.0 4,217,336 + PS417_19445 0.33 -0.0 4,217,337 - PS417_19445 0.33 +1.4 4,217,429 + PS417_19445 0.45 +0.1 4,217,429 + PS417_19445 0.45 +0.8 4,217,429 + PS417_19445 0.45 -0.3 4,217,429 + PS417_19445 0.45 -3.6 4,217,430 - PS417_19445 0.45 -0.1 4,217,460 - PS417_19445 0.49 -0.0 4,217,505 - PS417_19445 0.54 +4.3 4,217,523 - PS417_19445 0.57 +0.3 4,217,676 - PS417_19445 0.76 +1.1 4,217,711 + PS417_19445 0.81 -1.2 4,217,711 + PS417_19445 0.81 -0.2 4,217,753 + PS417_19445 0.86 -1.2 4,217,753 + +4.2 4,217,753 + PS417_19445 0.86 -0.3 4,217,753 + PS417_19445 0.86 +1.4 4,217,753 + PS417_19445 0.86 +2.4 4,217,754 - PS417_19445 0.86 -0.4 4,217,754 - PS417_19445 0.86 -0.1 4,217,769 - PS417_19445 0.88 -0.8 4,217,821 + +0.0 4,217,853 - +0.5 4,217,853 - -1.6 4,218,007 + -1.3 4,218,242 + PS417_19450 0.31 -1.5 4,218,242 + PS417_19450 0.31 +0.0 4,218,242 + PS417_19450 0.31 +2.0 4,218,243 - PS417_19450 0.31 +0.4 4,218,284 - PS417_19450 0.34 +0.4 4,218,284 - PS417_19450 0.34 -0.2 4,218,284 - PS417_19450 0.34 -0.2 4,218,825 + PS417_19450 0.82 -0.3 4,218,825 + PS417_19450 0.82 -2.3 4,218,825 + PS417_19450 0.82 -0.4
Or see this region's nucleotide sequence