Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_16155 and PS417_16160 overlap by 4 nucleotides PS417_16160 and PS417_16165 are separated by 245 nucleotides
PS417_16155: PS417_16155 - monooxygenase, at 3,542,287 to 3,543,612
_16155
PS417_16160: PS417_16160 - ABC transporter substrate-binding protein, at 3,543,609 to 3,544,547
_16160
PS417_16165: PS417_16165 - ABC transporter substrate-binding protein, at 3,544,793 to 3,545,737
_16165
Position (kb)
3543
3544
3545 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3543.213 kb on + strand, within PS417_16155 at 3543.214 kb on - strand, within PS417_16155 at 3543.287 kb on + strand, within PS417_16155 at 3543.287 kb on + strand, within PS417_16155 at 3543.288 kb on - strand, within PS417_16155 at 3543.341 kb on + strand, within PS417_16155 at 3543.500 kb on + strand at 3543.500 kb on + strand at 3543.680 kb on - strand at 3543.680 kb on - strand at 3543.739 kb on + strand, within PS417_16160 at 3543.932 kb on - strand, within PS417_16160 at 3544.256 kb on - strand, within PS417_16160 at 3544.259 kb on - strand, within PS417_16160 at 3544.259 kb on - strand at 3544.676 kb on + strand at 3544.677 kb on - strand at 3544.689 kb on + strand at 3544.734 kb on + strand at 3544.800 kb on + strand at 3544.800 kb on + strand at 3544.800 kb on + strand at 3544.801 kb on - strand at 3544.801 kb on - strand at 3544.801 kb on - strand at 3545.222 kb on + strand at 3545.420 kb on + strand, within PS417_16165 at 3545.421 kb on - strand, within PS417_16165 at 3545.430 kb on - strand at 3545.432 kb on + strand, within PS417_16165 at 3545.433 kb on - strand, within PS417_16165 at 3545.487 kb on - strand, within PS417_16165
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 3,543,213 + PS417_16155 0.70 -0.0 3,543,214 - PS417_16155 0.70 +0.3 3,543,287 + PS417_16155 0.75 +0.6 3,543,287 + PS417_16155 0.75 -0.3 3,543,288 - PS417_16155 0.75 -1.5 3,543,341 + PS417_16155 0.79 -0.8 3,543,500 + +0.0 3,543,500 + -0.6 3,543,680 - +2.0 3,543,680 - -0.3 3,543,739 + PS417_16160 0.14 -1.2 3,543,932 - PS417_16160 0.34 -1.2 3,544,256 - PS417_16160 0.69 -0.4 3,544,259 - PS417_16160 0.69 -1.6 3,544,259 - -0.6 3,544,676 + -0.5 3,544,677 - +0.3 3,544,689 + +0.5 3,544,734 + +0.8 3,544,800 + -1.9 3,544,800 + +0.4 3,544,800 + +0.4 3,544,801 - -0.2 3,544,801 - -0.7 3,544,801 - -0.7 3,545,222 + -0.2 3,545,420 + PS417_16165 0.66 +0.5 3,545,421 - PS417_16165 0.66 +2.5 3,545,430 - -0.2 3,545,432 + PS417_16165 0.68 -0.4 3,545,433 - PS417_16165 0.68 -1.0 3,545,487 - PS417_16165 0.73 -0.6
Or see this region's nucleotide sequence