Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00010 and PS417_00015 are separated by 20 nucleotides PS417_00015 and PS417_00020 are separated by 4 nucleotides
PS417_00010: PS417_00010 - DNA polymerase III subunit beta, at 1,781 to 2,884
_00010
PS417_00015: PS417_00015 - recombinase RecF, at 2,905 to 4,008
_00015
PS417_00020: PS417_00020 - DNA gyrase subunit B, at 4,013 to 6,430
_00020
Position (kb)
2
3
4
5 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2.902 kb on + strand at 2.902 kb on + strand at 2.902 kb on + strand at 2.903 kb on - strand at 2.903 kb on - strand at 3.147 kb on + strand, within PS417_00015 at 3.148 kb on - strand, within PS417_00015 at 3.148 kb on - strand, within PS417_00015 at 3.473 kb on + strand, within PS417_00015 at 3.473 kb on + strand, within PS417_00015 at 3.493 kb on + strand, within PS417_00015 at 3.493 kb on + strand, within PS417_00015 at 3.495 kb on + strand, within PS417_00015 at 3.495 kb on + strand, within PS417_00015 at 3.495 kb on + strand, within PS417_00015 at 3.684 kb on + strand, within PS417_00015 at 3.801 kb on + strand, within PS417_00015 at 3.801 kb on + strand, within PS417_00015 at 3.801 kb on + strand, within PS417_00015 at 3.801 kb on + strand, within PS417_00015 at 3.805 kb on + strand, within PS417_00015 at 3.878 kb on + strand, within PS417_00015 at 3.878 kb on + strand, within PS417_00015 at 3.917 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 2,902 + -1.6 2,902 + -1.9 2,902 + -0.5 2,903 - -1.0 2,903 - -0.6 3,147 + PS417_00015 0.22 -0.5 3,148 - PS417_00015 0.22 -0.6 3,148 - PS417_00015 0.22 +1.5 3,473 + PS417_00015 0.51 +1.2 3,473 + PS417_00015 0.51 +0.5 3,493 + PS417_00015 0.53 -0.5 3,493 + PS417_00015 0.53 +1.0 3,495 + PS417_00015 0.53 -1.6 3,495 + PS417_00015 0.53 -1.0 3,495 + PS417_00015 0.53 -0.0 3,684 + PS417_00015 0.71 -2.5 3,801 + PS417_00015 0.81 +0.3 3,801 + PS417_00015 0.81 +2.1 3,801 + PS417_00015 0.81 +0.3 3,801 + PS417_00015 0.81 +1.1 3,805 + PS417_00015 0.82 +0.5 3,878 + PS417_00015 0.88 -2.4 3,878 + PS417_00015 0.88 +1.8 3,917 + -0.6
Or see this region's nucleotide sequence