Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_14990 and PS417_14995 are separated by 210 nucleotides PS417_14995 and PS417_15005 are separated by 994 nucleotides
PS417_14990: PS417_14990 - bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase, at 3,276,395 to 3,279,004
_14990
PS417_14995: PS417_14995 - serine/threonine protein kinase, at 3,279,215 to 3,280,492
_14995
PS417_15005: PS417_15005 - hypothetical protein, at 3,281,487 to 3,281,930
_15005
Position (kb)
3279
3280
3281 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3279.146 kb on - strand at 3279.298 kb on - strand at 3279.330 kb on + strand at 3279.330 kb on + strand at 3279.531 kb on + strand, within PS417_14995 at 3279.531 kb on + strand, within PS417_14995 at 3279.778 kb on - strand, within PS417_14995 at 3279.869 kb on - strand, within PS417_14995 at 3279.940 kb on - strand, within PS417_14995 at 3280.137 kb on + strand, within PS417_14995 at 3280.138 kb on - strand, within PS417_14995 at 3280.170 kb on + strand, within PS417_14995 at 3280.512 kb on + strand at 3280.512 kb on + strand at 3280.513 kb on - strand at 3280.513 kb on - strand at 3280.513 kb on - strand at 3280.596 kb on - strand at 3280.786 kb on + strand at 3280.844 kb on + strand at 3281.143 kb on + strand at 3281.144 kb on - strand at 3281.144 kb on - strand at 3281.144 kb on - strand at 3281.389 kb on + strand at 3281.389 kb on + strand at 3281.389 kb on + strand at 3281.390 kb on - strand at 3281.390 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 3,279,146 - -1.1 3,279,298 - +2.4 3,279,330 + -0.2 3,279,330 + +1.1 3,279,531 + PS417_14995 0.25 -0.7 3,279,531 + PS417_14995 0.25 -0.9 3,279,778 - PS417_14995 0.44 -3.0 3,279,869 - PS417_14995 0.51 -1.8 3,279,940 - PS417_14995 0.57 -0.2 3,280,137 + PS417_14995 0.72 -2.8 3,280,138 - PS417_14995 0.72 +0.1 3,280,170 + PS417_14995 0.75 -1.5 3,280,512 + +0.1 3,280,512 + -0.7 3,280,513 - -0.9 3,280,513 - -0.4 3,280,513 - +1.6 3,280,596 - +0.3 3,280,786 + +0.0 3,280,844 + +0.2 3,281,143 + -1.6 3,281,144 - -0.5 3,281,144 - -0.2 3,281,144 - +1.6 3,281,389 + +0.5 3,281,389 + -0.3 3,281,389 + +0.3 3,281,390 - -1.1 3,281,390 - +0.4
Or see this region's nucleotide sequence