Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_14930 and PS417_14935 are separated by 28 nucleotides PS417_14935 and PS417_14940 are separated by 131 nucleotides PS417_14940 and PS417_14945 are separated by 116 nucleotides
PS417_14930: PS417_14930 - acyl-CoA dehydrogenase, at 3,260,489 to 3,261,652
_14930
PS417_14935: PS417_14935 - transposase, at 3,261,681 to 3,262,499
_14935
PS417_14940: PS417_14940 - LysR family transcriptional regulator, at 3,262,631 to 3,263,521
_14940
PS417_14945: PS417_14945 - beta-lactamase, at 3,263,638 to 3,265,452
_14945
Position (kb)
3262
3263
3264 Strain fitness (log2 ratio)
-2
-1
0
1 at 3261.815 kb on - strand, within PS417_14935 at 3261.943 kb on + strand, within PS417_14935 at 3262.126 kb on + strand, within PS417_14935 at 3263.044 kb on - strand, within PS417_14940 at 3263.741 kb on - strand at 3264.020 kb on - strand, within PS417_14945 at 3264.020 kb on - strand, within PS417_14945 at 3264.140 kb on - strand, within PS417_14945 at 3264.140 kb on - strand, within PS417_14945 at 3264.211 kb on + strand, within PS417_14945 at 3264.457 kb on + strand at 3264.471 kb on - strand, within PS417_14945 at 3264.488 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 3,261,815 - PS417_14935 0.16 +1.2 3,261,943 + PS417_14935 0.32 +1.2 3,262,126 + PS417_14935 0.54 -0.3 3,263,044 - PS417_14940 0.46 -1.1 3,263,741 - -0.2 3,264,020 - PS417_14945 0.21 -0.7 3,264,020 - PS417_14945 0.21 +0.4 3,264,140 - PS417_14945 0.28 -1.8 3,264,140 - PS417_14945 0.28 -0.7 3,264,211 + PS417_14945 0.32 -0.2 3,264,457 + -0.6 3,264,471 - PS417_14945 0.46 -0.2 3,264,488 - -0.6
Or see this region's nucleotide sequence