Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_14530 and PS417_14535 are separated by 138 nucleotides PS417_14535 and PS417_14540 overlap by 1 nucleotides PS417_14540 and PS417_14545 overlap by 28 nucleotides
PS417_14530: PS417_14530 - acetylglucosaminylphosphatidylinositol deacetylase, at 3,176,743 to 3,177,504
_14530
PS417_14535: PS417_14535 - alpha/beta hydrolase, at 3,177,643 to 3,178,677
_14535
PS417_14540: PS417_14540 - cytochrome C oxidase subunit III, at 3,178,677 to 3,179,156
_14540
PS417_14545: PS417_14545 - amino acid transporter, at 3,179,129 to 3,180,526
_14545
Position (kb)
3177
3178
3179 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3176.827 kb on - strand, within PS417_14530 at 3177.274 kb on + strand, within PS417_14530 at 3177.274 kb on + strand, within PS417_14530 at 3177.275 kb on - strand, within PS417_14530 at 3177.516 kb on + strand at 3177.545 kb on + strand at 3177.546 kb on - strand at 3177.556 kb on + strand at 3177.587 kb on + strand at 3177.902 kb on - strand, within PS417_14535 at 3177.902 kb on - strand, within PS417_14535 at 3177.902 kb on - strand, within PS417_14535 at 3177.902 kb on - strand, within PS417_14535 at 3178.556 kb on - strand, within PS417_14535 at 3178.579 kb on + strand at 3178.579 kb on + strand at 3178.691 kb on + strand at 3178.691 kb on + strand at 3178.692 kb on - strand at 3179.017 kb on - strand, within PS417_14540 at 3179.361 kb on + strand, within PS417_14545 at 3179.361 kb on + strand, within PS417_14545 at 3179.362 kb on - strand, within PS417_14545 at 3179.386 kb on + strand, within PS417_14545 at 3179.387 kb on - strand, within PS417_14545 at 3179.387 kb on - strand, within PS417_14545 at 3179.492 kb on - strand, within PS417_14545 at 3179.507 kb on - strand, within PS417_14545
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 3,176,827 - PS417_14530 0.11 -0.3 3,177,274 + PS417_14530 0.70 -0.1 3,177,274 + PS417_14530 0.70 -2.8 3,177,275 - PS417_14530 0.70 -0.2 3,177,516 + -0.6 3,177,545 + +0.2 3,177,546 - +0.2 3,177,556 + -0.4 3,177,587 + +1.9 3,177,902 - PS417_14535 0.25 -0.9 3,177,902 - PS417_14535 0.25 +2.0 3,177,902 - PS417_14535 0.25 -0.4 3,177,902 - PS417_14535 0.25 -1.2 3,178,556 - PS417_14535 0.88 -1.6 3,178,579 + +1.2 3,178,579 + -1.9 3,178,691 + +0.6 3,178,691 + +0.4 3,178,692 - +2.4 3,179,017 - PS417_14540 0.71 +0.6 3,179,361 + PS417_14545 0.17 -0.7 3,179,361 + PS417_14545 0.17 -0.3 3,179,362 - PS417_14545 0.17 +3.0 3,179,386 + PS417_14545 0.18 +0.1 3,179,387 - PS417_14545 0.18 +0.9 3,179,387 - PS417_14545 0.18 +0.3 3,179,492 - PS417_14545 0.26 +0.1 3,179,507 - PS417_14545 0.27 -1.6
Or see this region's nucleotide sequence