Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01355 and PS417_01360 are separated by 202 nucleotides PS417_01360 and PS417_01365 are separated by 20 nucleotides PS417_01365 and PS417_01370 are separated by 3 nucleotides
PS417_01355: PS417_01355 - membrane protein, at 296,093 to 297,409
_01355
PS417_01360: PS417_01360 - 4-oxalocrotonate tautomerase, at 297,612 to 297,815
_01360
PS417_01365: PS417_01365 - 3-oxoacyl-ACP reductase, at 297,836 to 298,537
_01365
PS417_01370: PS417_01370 - peptidase M48, at 298,541 to 300,118
_01370
Position (kb) 297
298
299 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 297.086 kb on + strand, within PS417_01355 at 297.176 kb on + strand, within PS417_01355 at 297.177 kb on - strand, within PS417_01355 at 297.189 kb on - strand, within PS417_01355 at 297.218 kb on - strand, within PS417_01355 at 297.266 kb on + strand, within PS417_01355 at 297.267 kb on - strand, within PS417_01355 at 297.273 kb on + strand, within PS417_01355 at 297.273 kb on + strand, within PS417_01355 at 297.274 kb on - strand, within PS417_01355 at 297.274 kb on - strand, within PS417_01355 at 297.350 kb on + strand at 297.351 kb on - strand at 297.407 kb on + strand at 297.408 kb on - strand at 297.408 kb on - strand at 297.469 kb on + strand at 297.518 kb on + strand at 297.518 kb on + strand at 297.519 kb on - strand at 297.555 kb on + strand at 297.555 kb on + strand at 297.555 kb on + strand at 297.651 kb on - strand, within PS417_01360 at 297.771 kb on + strand, within PS417_01360 at 298.100 kb on + strand at 298.154 kb on + strand, within PS417_01365 at 298.154 kb on + strand, within PS417_01365 at 298.509 kb on + strand at 298.510 kb on - strand at 298.579 kb on + strand at 298.579 kb on + strand at 298.656 kb on - strand at 298.860 kb on + strand, within PS417_01370 at 298.971 kb on + strand, within PS417_01370 at 298.971 kb on + strand, within PS417_01370 at 299.122 kb on + strand, within PS417_01370 at 299.122 kb on + strand, within PS417_01370 at 299.122 kb on + strand, within PS417_01370 at 299.122 kb on + strand, within PS417_01370 at 299.123 kb on - strand, within PS417_01370 at 299.455 kb on - strand, within PS417_01370
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 297,086 + PS417_01355 0.75 +0.8 297,176 + PS417_01355 0.82 -2.3 297,177 - PS417_01355 0.82 -0.5 297,189 - PS417_01355 0.83 +0.0 297,218 - PS417_01355 0.85 -2.8 297,266 + PS417_01355 0.89 -1.9 297,267 - PS417_01355 0.89 -1.5 297,273 + PS417_01355 0.90 -0.5 297,273 + PS417_01355 0.90 -1.0 297,274 - PS417_01355 0.90 -3.0 297,274 - PS417_01355 0.90 -0.6 297,350 + +0.9 297,351 - +3.1 297,407 + -0.4 297,408 - -0.5 297,408 - -2.8 297,469 + -0.8 297,518 + -1.0 297,518 + +1.6 297,519 - -0.6 297,555 + -0.8 297,555 + +0.4 297,555 + +0.1 297,651 - PS417_01360 0.19 +1.7 297,771 + PS417_01360 0.78 -0.2 298,100 + +0.5 298,154 + PS417_01365 0.45 -2.7 298,154 + PS417_01365 0.45 +2.5 298,509 + -0.1 298,510 - -0.2 298,579 + -0.8 298,579 + -1.1 298,656 - +0.4 298,860 + PS417_01370 0.20 -0.4 298,971 + PS417_01370 0.27 -0.2 298,971 + PS417_01370 0.27 -0.1 299,122 + PS417_01370 0.37 -0.3 299,122 + PS417_01370 0.37 -2.1 299,122 + PS417_01370 0.37 -0.2 299,122 + PS417_01370 0.37 +0.2 299,123 - PS417_01370 0.37 +0.2 299,455 - PS417_01370 0.58 -0.7
Or see this region's nucleotide sequence