Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12995 and PS417_13000 are separated by 322 nucleotides PS417_13000 and PS417_13005 are separated by 13 nucleotides PS417_13005 and PS417_13010 are separated by 14 nucleotides PS417_13010 and PS417_13015 overlap by 4 nucleotides
PS417_12995: PS417_12995 - MFS transporter permease, at 2,838,909 to 2,840,219
_12995
PS417_13000: PS417_13000 - isochorismatase, at 2,840,542 to 2,841,174
_13000
PS417_13005: PS417_13005 - Asp/Glu racemase, at 2,841,188 to 2,841,940
_13005
PS417_13010: PS417_13010 - alpha/beta hydrolase, at 2,841,955 to 2,842,752
_13010
PS417_13015: PS417_13015 - 6-hydroxynicotinate 3-monooxygenase, at 2,842,749 to 2,843,882
_13015
Position (kb)
2841
2842 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2840.242 kb on + strand at 2840.288 kb on + strand at 2840.289 kb on - strand at 2840.363 kb on + strand at 2840.364 kb on - strand at 2840.364 kb on - strand at 2840.518 kb on + strand at 2840.533 kb on - strand at 2840.533 kb on - strand at 2840.533 kb on - strand at 2840.535 kb on + strand at 2840.543 kb on + strand at 2840.594 kb on + strand at 2840.595 kb on - strand at 2841.034 kb on + strand at 2841.192 kb on + strand at 2841.498 kb on + strand, within PS417_13005 at 2841.498 kb on + strand, within PS417_13005 at 2841.499 kb on - strand, within PS417_13005 at 2841.794 kb on + strand, within PS417_13005 at 2841.956 kb on + strand at 2842.326 kb on - strand, within PS417_13010 at 2842.469 kb on + strand, within PS417_13010 at 2842.470 kb on - strand, within PS417_13010 at 2842.792 kb on + strand at 2842.798 kb on + strand at 2842.892 kb on - strand, within PS417_13015
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 2,840,242 + -0.9 2,840,288 + -0.8 2,840,289 - -1.3 2,840,363 + +0.9 2,840,364 - -1.6 2,840,364 - -0.2 2,840,518 + -1.6 2,840,533 - +1.9 2,840,533 - +0.2 2,840,533 - +0.5 2,840,535 + +1.9 2,840,543 + -0.6 2,840,594 + +0.7 2,840,595 - -1.8 2,841,034 + +1.4 2,841,192 + +0.9 2,841,498 + PS417_13005 0.41 -0.1 2,841,498 + PS417_13005 0.41 +0.7 2,841,499 - PS417_13005 0.41 -1.2 2,841,794 + PS417_13005 0.80 -1.4 2,841,956 + +0.3 2,842,326 - PS417_13010 0.46 -2.0 2,842,469 + PS417_13010 0.64 -1.2 2,842,470 - PS417_13010 0.65 -0.7 2,842,792 + +1.0 2,842,798 + +0.9 2,842,892 - PS417_13015 0.13 -0.6
Or see this region's nucleotide sequence