Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12995 and PS417_13000 are separated by 322 nucleotides PS417_13000 and PS417_13005 are separated by 13 nucleotides PS417_13005 and PS417_13010 are separated by 14 nucleotides
PS417_12995: PS417_12995 - MFS transporter permease, at 2,838,909 to 2,840,219
_12995
PS417_13000: PS417_13000 - isochorismatase, at 2,840,542 to 2,841,174
_13000
PS417_13005: PS417_13005 - Asp/Glu racemase, at 2,841,188 to 2,841,940
_13005
PS417_13010: PS417_13010 - alpha/beta hydrolase, at 2,841,955 to 2,842,752
_13010
Position (kb)
2840
2841
2842 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2839.586 kb on - strand, within PS417_12995 at 2839.808 kb on - strand, within PS417_12995 at 2839.810 kb on + strand, within PS417_12995 at 2839.810 kb on + strand, within PS417_12995 at 2839.867 kb on + strand, within PS417_12995 at 2839.867 kb on + strand, within PS417_12995 at 2839.998 kb on - strand, within PS417_12995 at 2839.998 kb on - strand, within PS417_12995 at 2840.000 kb on - strand, within PS417_12995 at 2840.052 kb on + strand, within PS417_12995 at 2840.052 kb on + strand, within PS417_12995 at 2840.053 kb on - strand, within PS417_12995 at 2840.053 kb on - strand, within PS417_12995 at 2840.053 kb on - strand, within PS417_12995 at 2840.105 kb on - strand at 2840.105 kb on - strand at 2840.117 kb on - strand at 2840.242 kb on + strand at 2840.288 kb on + strand at 2840.289 kb on - strand at 2840.363 kb on + strand at 2840.364 kb on - strand at 2840.364 kb on - strand at 2840.518 kb on + strand at 2840.533 kb on - strand at 2840.533 kb on - strand at 2840.533 kb on - strand at 2840.535 kb on + strand at 2840.543 kb on + strand at 2840.594 kb on + strand at 2840.595 kb on - strand at 2841.034 kb on + strand at 2841.192 kb on + strand at 2841.498 kb on + strand, within PS417_13005 at 2841.498 kb on + strand, within PS417_13005 at 2841.499 kb on - strand, within PS417_13005 at 2841.794 kb on + strand, within PS417_13005 at 2841.956 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 2,839,586 - PS417_12995 0.52 -0.3 2,839,808 - PS417_12995 0.69 -0.4 2,839,810 + PS417_12995 0.69 -0.1 2,839,810 + PS417_12995 0.69 +0.2 2,839,867 + PS417_12995 0.73 +0.0 2,839,867 + PS417_12995 0.73 +2.6 2,839,998 - PS417_12995 0.83 +1.0 2,839,998 - PS417_12995 0.83 +0.2 2,840,000 - PS417_12995 0.83 -0.1 2,840,052 + PS417_12995 0.87 +1.6 2,840,052 + PS417_12995 0.87 -0.6 2,840,053 - PS417_12995 0.87 -1.2 2,840,053 - PS417_12995 0.87 -0.4 2,840,053 - PS417_12995 0.87 -0.8 2,840,105 - -0.2 2,840,105 - -0.1 2,840,117 - +3.9 2,840,242 + -0.9 2,840,288 + -0.8 2,840,289 - -1.3 2,840,363 + +0.9 2,840,364 - -1.6 2,840,364 - -0.2 2,840,518 + -1.6 2,840,533 - +1.9 2,840,533 - +0.2 2,840,533 - +0.5 2,840,535 + +1.9 2,840,543 + -0.6 2,840,594 + +0.7 2,840,595 - -1.8 2,841,034 + +1.4 2,841,192 + +0.9 2,841,498 + PS417_13005 0.41 -0.1 2,841,498 + PS417_13005 0.41 +0.7 2,841,499 - PS417_13005 0.41 -1.2 2,841,794 + PS417_13005 0.80 -1.4 2,841,956 + +0.3
Or see this region's nucleotide sequence