Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12965 and PS417_12970 are separated by 59 nucleotides PS417_12970 and PS417_12975 overlap by 4 nucleotides PS417_12975 and PS417_12980 overlap by 4 nucleotides PS417_12980 and PS417_12985 are separated by 131 nucleotides
PS417_12965: PS417_12965 - aminotransferase, at 2,833,469 to 2,834,620
_12965
PS417_12970: PS417_12970 - FMN-dependent NADH-azoreductase, at 2,834,680 to 2,835,273
_12970
PS417_12975: PS417_12975 - GCN5 family acetyltransferase, at 2,835,270 to 2,835,710
_12975
PS417_12980: PS417_12980 - alkylhydroperoxidase, at 2,835,707 to 2,836,180
_12980
PS417_12985: PS417_12985 - DNA-binding protein, at 2,836,312 to 2,837,739
_12985
Position (kb)
2835
2836 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2834.429 kb on + strand, within PS417_12965 at 2834.429 kb on + strand at 2834.631 kb on + strand at 2834.682 kb on - strand at 2834.684 kb on - strand at 2834.805 kb on + strand, within PS417_12970 at 2834.806 kb on - strand, within PS417_12970 at 2834.825 kb on + strand, within PS417_12970 at 2834.825 kb on + strand, within PS417_12970 at 2834.826 kb on - strand, within PS417_12970 at 2834.826 kb on - strand, within PS417_12970 at 2835.004 kb on - strand, within PS417_12970 at 2835.120 kb on - strand, within PS417_12970 at 2835.155 kb on + strand, within PS417_12970 at 2835.350 kb on + strand, within PS417_12975 at 2835.466 kb on - strand, within PS417_12975 at 2835.478 kb on + strand, within PS417_12975 at 2835.479 kb on - strand, within PS417_12975 at 2835.482 kb on + strand, within PS417_12975 at 2835.483 kb on - strand, within PS417_12975 at 2835.493 kb on + strand, within PS417_12975 at 2835.494 kb on - strand, within PS417_12975 at 2835.494 kb on - strand, within PS417_12975 at 2836.110 kb on + strand, within PS417_12980 at 2836.331 kb on - strand at 2836.539 kb on - strand, within PS417_12985
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 2,834,429 + PS417_12965 0.83 -0.6 2,834,429 + +0.4 2,834,631 + +0.2 2,834,682 - -1.0 2,834,684 - +0.9 2,834,805 + PS417_12970 0.21 -1.9 2,834,806 - PS417_12970 0.21 -2.1 2,834,825 + PS417_12970 0.24 +2.7 2,834,825 + PS417_12970 0.24 -0.4 2,834,826 - PS417_12970 0.25 +1.6 2,834,826 - PS417_12970 0.25 -1.1 2,835,004 - PS417_12970 0.55 -0.4 2,835,120 - PS417_12970 0.74 +1.0 2,835,155 + PS417_12970 0.80 +1.8 2,835,350 + PS417_12975 0.18 -2.4 2,835,466 - PS417_12975 0.44 -1.6 2,835,478 + PS417_12975 0.47 -0.8 2,835,479 - PS417_12975 0.47 -0.6 2,835,482 + PS417_12975 0.48 -1.2 2,835,483 - PS417_12975 0.48 -0.3 2,835,493 + PS417_12975 0.51 -1.4 2,835,494 - PS417_12975 0.51 -0.5 2,835,494 - PS417_12975 0.51 -0.4 2,836,110 + PS417_12980 0.85 -1.2 2,836,331 - +1.1 2,836,539 - PS417_12985 0.16 -2.0
Or see this region's nucleotide sequence