Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12940 and PS417_12945 are separated by 37 nucleotides PS417_12945 and PS417_12950 overlap by 4 nucleotides PS417_12950 and PS417_12955 are separated by 10 nucleotides
PS417_12940: PS417_12940 - sugar ABC transporter substrate-binding protein, at 2,829,281 to 2,830,207
_12940
PS417_12945: PS417_12945 - sulfonate ABC transporter ATP-binding protein, at 2,830,245 to 2,830,961
_12945
PS417_12950: PS417_12950 - ABC transporter permease, at 2,830,958 to 2,831,779
_12950
PS417_12955: PS417_12955 - sulfonate ABC transporter substrate-binding protein, at 2,831,790 to 2,832,746
_12955
Position (kb)
2830
2831
2832 Strain fitness (log2 ratio)
-2
-1
0
1 at 2830.048 kb on - strand, within PS417_12940 at 2830.048 kb on - strand, within PS417_12940 at 2830.170 kb on - strand at 2830.250 kb on + strand at 2830.402 kb on + strand, within PS417_12945 at 2830.672 kb on + strand, within PS417_12945 at 2830.672 kb on + strand, within PS417_12945 at 2830.929 kb on + strand at 2831.791 kb on + strand at 2831.792 kb on - strand at 2832.046 kb on + strand, within PS417_12955 at 2832.118 kb on + strand, within PS417_12955 at 2832.428 kb on - strand, within PS417_12955 at 2832.434 kb on - strand, within PS417_12955 at 2832.434 kb on - strand, within PS417_12955 at 2832.434 kb on - strand, within PS417_12955 at 2832.434 kb on - strand, within PS417_12955 at 2832.471 kb on + strand, within PS417_12955 at 2832.524 kb on - strand, within PS417_12955 at 2832.632 kb on - strand, within PS417_12955 at 2832.634 kb on + strand, within PS417_12955 at 2832.723 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 2,830,048 - PS417_12940 0.83 -1.5 2,830,048 - PS417_12940 0.83 -0.3 2,830,170 - -0.5 2,830,250 + +0.7 2,830,402 + PS417_12945 0.22 -0.0 2,830,672 + PS417_12945 0.60 -0.8 2,830,672 + PS417_12945 0.60 -0.8 2,830,929 + +0.2 2,831,791 + -0.3 2,831,792 - +0.4 2,832,046 + PS417_12955 0.27 -1.0 2,832,118 + PS417_12955 0.34 -1.2 2,832,428 - PS417_12955 0.67 -0.4 2,832,434 - PS417_12955 0.67 -0.4 2,832,434 - PS417_12955 0.67 +0.0 2,832,434 - PS417_12955 0.67 -0.9 2,832,434 - PS417_12955 0.67 +1.0 2,832,471 + PS417_12955 0.71 +0.2 2,832,524 - PS417_12955 0.77 -1.1 2,832,632 - PS417_12955 0.88 +0.4 2,832,634 + PS417_12955 0.88 -2.7 2,832,723 + -0.4
Or see this region's nucleotide sequence