Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12510 and PS417_12515 are separated by 210 nucleotides PS417_12515 and PS417_12520 are separated by 33 nucleotides PS417_12520 and PS417_12525 are separated by 163 nucleotides PS417_12525 and PS417_12530 overlap by 8 nucleotides
PS417_12510: PS417_12510 - hypothetical protein, at 2,718,717 to 2,723,834
_12510
PS417_12515: PS417_12515 - lactate dehydrogenase, at 2,724,045 to 2,725,082
_12515
PS417_12520: PS417_12520 - hypothetical protein, at 2,725,116 to 2,725,436
_12520
PS417_12525: PS417_12525 - hypothetical protein, at 2,725,600 to 2,725,854
_12525
PS417_12530: PS417_12530 - LysR family transcriptional regulator, at 2,725,847 to 2,726,740
_12530
Position (kb)
2724
2725
2726 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2723.081 kb on - strand, within PS417_12510 at 2723.131 kb on + strand, within PS417_12510 at 2723.383 kb on - strand at 2723.517 kb on + strand at 2723.518 kb on - strand at 2723.518 kb on - strand at 2723.584 kb on + strand at 2723.584 kb on + strand at 2723.584 kb on + strand at 2723.585 kb on - strand at 2723.585 kb on - strand at 2723.824 kb on - strand at 2723.835 kb on - strand at 2723.835 kb on - strand at 2723.835 kb on - strand at 2723.922 kb on + strand at 2723.922 kb on + strand at 2723.926 kb on + strand at 2723.927 kb on - strand at 2723.927 kb on - strand at 2723.927 kb on - strand at 2723.927 kb on - strand at 2723.927 kb on - strand at 2723.927 kb on - strand at 2723.983 kb on + strand at 2723.984 kb on - strand at 2724.007 kb on - strand at 2724.007 kb on - strand at 2724.093 kb on + strand at 2724.163 kb on + strand, within PS417_12515 at 2724.235 kb on - strand, within PS417_12515 at 2724.235 kb on - strand, within PS417_12515 at 2725.030 kb on + strand at 2725.122 kb on - strand at 2725.202 kb on - strand, within PS417_12520 at 2725.202 kb on - strand, within PS417_12520 at 2725.202 kb on - strand, within PS417_12520 at 2725.557 kb on + strand at 2725.557 kb on + strand at 2725.558 kb on - strand at 2725.558 kb on - strand at 2725.558 kb on - strand at 2725.558 kb on - strand at 2725.558 kb on - strand at 2725.564 kb on + strand at 2725.564 kb on + strand at 2725.565 kb on - strand at 2725.565 kb on - strand at 2725.565 kb on - strand at 2725.565 kb on - strand at 2725.565 kb on - strand at 2725.577 kb on - strand at 2725.592 kb on - strand at 2725.592 kb on - strand at 2725.669 kb on + strand, within PS417_12525 at 2725.669 kb on + strand, within PS417_12525 at 2725.669 kb on + strand, within PS417_12525 at 2725.744 kb on - strand, within PS417_12525 at 2725.852 kb on + strand at 2725.852 kb on + strand at 2725.853 kb on - strand at 2726.081 kb on + strand, within PS417_12530
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 2,723,081 - PS417_12510 0.85 +0.4 2,723,131 + PS417_12510 0.86 +0.8 2,723,383 - +1.0 2,723,517 + +1.2 2,723,518 - +0.0 2,723,518 - +0.1 2,723,584 + +1.6 2,723,584 + -0.0 2,723,584 + +1.0 2,723,585 - -0.4 2,723,585 - -0.6 2,723,824 - -2.9 2,723,835 - -0.9 2,723,835 - +0.1 2,723,835 - -0.7 2,723,922 + -0.9 2,723,922 + -1.7 2,723,926 + +1.0 2,723,927 - +0.3 2,723,927 - -0.2 2,723,927 - -1.1 2,723,927 - +0.5 2,723,927 - -0.8 2,723,927 - +0.4 2,723,983 + -0.9 2,723,984 - -0.8 2,724,007 - -0.2 2,724,007 - +0.8 2,724,093 + +2.6 2,724,163 + PS417_12515 0.11 -0.8 2,724,235 - PS417_12515 0.18 -2.4 2,724,235 - PS417_12515 0.18 -2.4 2,725,030 + -0.9 2,725,122 - +1.4 2,725,202 - PS417_12520 0.27 -1.2 2,725,202 - PS417_12520 0.27 -1.0 2,725,202 - PS417_12520 0.27 -0.5 2,725,557 + +1.0 2,725,557 + -1.4 2,725,558 - -1.6 2,725,558 - -1.9 2,725,558 - -0.2 2,725,558 - -0.9 2,725,558 - +1.4 2,725,564 + -0.2 2,725,564 + -0.1 2,725,565 - +1.5 2,725,565 - -0.1 2,725,565 - -1.6 2,725,565 - +0.3 2,725,565 - -0.7 2,725,577 - -0.4 2,725,592 - -0.4 2,725,592 - +1.6 2,725,669 + PS417_12525 0.27 +1.8 2,725,669 + PS417_12525 0.27 +0.5 2,725,669 + PS417_12525 0.27 -0.6 2,725,744 - PS417_12525 0.56 -0.2 2,725,852 + -0.4 2,725,852 + +0.2 2,725,853 - -1.1 2,726,081 + PS417_12530 0.26 +0.7
Or see this region's nucleotide sequence