Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12365 and PS417_12370 are separated by 144 nucleotides PS417_12370 and PS417_12375 overlap by 1 nucleotides PS417_12375 and PS417_12380 overlap by 4 nucleotides
PS417_12365: PS417_12365 - cobalt chelatase, at 2,689,905 to 2,690,852
_12365
PS417_12370: PS417_12370 - inorganic polyphosphate kinase, at 2,690,997 to 2,691,887
_12370
PS417_12375: PS417_12375 - serine/threonine protein phosphatase, at 2,691,887 to 2,692,858
_12375
PS417_12380: PS417_12380 - peptidase S54, at 2,692,855 to 2,693,724
_12380
Position (kb)
2690
2691
2692 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2690.240 kb on - strand, within PS417_12365 at 2690.283 kb on + strand, within PS417_12365 at 2690.283 kb on + strand, within PS417_12365 at 2690.284 kb on - strand, within PS417_12365 at 2690.284 kb on - strand, within PS417_12365 at 2690.284 kb on - strand, within PS417_12365 at 2690.441 kb on - strand, within PS417_12365 at 2690.599 kb on + strand, within PS417_12365 at 2690.600 kb on - strand, within PS417_12365 at 2690.872 kb on - strand at 2690.897 kb on + strand at 2691.909 kb on - strand at 2692.438 kb on + strand, within PS417_12375 at 2692.438 kb on + strand, within PS417_12375 at 2692.760 kb on + strand, within PS417_12375 at 2692.871 kb on + strand at 2692.874 kb on + strand at 2692.874 kb on + strand at 2692.875 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 2,690,240 - PS417_12365 0.35 +1.4 2,690,283 + PS417_12365 0.40 -0.9 2,690,283 + PS417_12365 0.40 -1.0 2,690,284 - PS417_12365 0.40 +1.2 2,690,284 - PS417_12365 0.40 +0.3 2,690,284 - PS417_12365 0.40 +1.9 2,690,441 - PS417_12365 0.57 +1.6 2,690,599 + PS417_12365 0.73 +0.9 2,690,600 - PS417_12365 0.73 +0.6 2,690,872 - +0.2 2,690,897 + +0.4 2,691,909 - -0.9 2,692,438 + PS417_12375 0.57 -0.1 2,692,438 + PS417_12375 0.57 -1.2 2,692,760 + PS417_12375 0.90 -1.5 2,692,871 + -1.8 2,692,874 + -0.2 2,692,874 + -0.7 2,692,875 - +0.0
Or see this region's nucleotide sequence