Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12355 and PS417_12360 are separated by 53 nucleotides PS417_12360 and PS417_12365 are separated by 114 nucleotides PS417_12365 and PS417_12370 are separated by 144 nucleotides
PS417_12355: PS417_12355 - magnesium chelatase, at 2,688,136 to 2,689,134
_12355
PS417_12360: PS417_12360 - magnesium chelatase, at 2,689,188 to 2,689,790
_12360
PS417_12365: PS417_12365 - cobalt chelatase, at 2,689,905 to 2,690,852
_12365
PS417_12370: PS417_12370 - inorganic polyphosphate kinase, at 2,690,997 to 2,691,887
_12370
Position (kb)
2689
2690
2691 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2688.939 kb on + strand, within PS417_12355 at 2688.940 kb on - strand, within PS417_12355 at 2689.294 kb on - strand, within PS417_12360 at 2689.775 kb on + strand at 2689.789 kb on - strand at 2689.890 kb on + strand at 2689.891 kb on - strand at 2689.891 kb on - strand at 2690.240 kb on - strand, within PS417_12365 at 2690.283 kb on + strand, within PS417_12365 at 2690.283 kb on + strand, within PS417_12365 at 2690.284 kb on - strand, within PS417_12365 at 2690.284 kb on - strand, within PS417_12365 at 2690.284 kb on - strand, within PS417_12365 at 2690.441 kb on - strand, within PS417_12365 at 2690.599 kb on + strand, within PS417_12365 at 2690.600 kb on - strand, within PS417_12365 at 2690.872 kb on - strand at 2690.897 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 2,688,939 + PS417_12355 0.80 -1.2 2,688,940 - PS417_12355 0.80 -1.0 2,689,294 - PS417_12360 0.18 -1.4 2,689,775 + +0.1 2,689,789 - +0.0 2,689,890 + -1.3 2,689,891 - +1.5 2,689,891 - +3.7 2,690,240 - PS417_12365 0.35 +1.4 2,690,283 + PS417_12365 0.40 -0.9 2,690,283 + PS417_12365 0.40 -1.0 2,690,284 - PS417_12365 0.40 +1.2 2,690,284 - PS417_12365 0.40 +0.3 2,690,284 - PS417_12365 0.40 +1.9 2,690,441 - PS417_12365 0.57 +1.6 2,690,599 + PS417_12365 0.73 +0.9 2,690,600 - PS417_12365 0.73 +0.6 2,690,872 - +0.2 2,690,897 + +0.4
Or see this region's nucleotide sequence