Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11600 and PS417_11605 are separated by 10 nucleotides PS417_11605 and PS417_11610 overlap by 4 nucleotides PS417_11610 and PS417_11615 are separated by 10 nucleotides
PS417_11600: PS417_11600 - urea ABC transporter permease, at 2,495,361 to 2,496,278
_11600
PS417_11605: PS417_11605 - urea ABC transporter permease, at 2,496,289 to 2,497,452
_11605
PS417_11610: PS417_11610 - urea ABC transporter ATP-binding protein, at 2,497,449 to 2,498,207
_11610
PS417_11615: PS417_11615 - urea ABC transporter ATP-binding protein, at 2,498,218 to 2,498,907
_11615
Position (kb)
2496
2497
2498 Strain fitness (log2 ratio)
-2
-1
0
1 at 2495.532 kb on + strand, within PS417_11600 at 2495.532 kb on + strand, within PS417_11600 at 2495.533 kb on - strand, within PS417_11600 at 2495.580 kb on + strand, within PS417_11600 at 2495.667 kb on + strand, within PS417_11600 at 2495.668 kb on - strand, within PS417_11600 at 2495.668 kb on - strand, within PS417_11600 at 2496.070 kb on - strand, within PS417_11600 at 2496.203 kb on - strand at 2496.277 kb on - strand at 2496.424 kb on + strand at 2496.424 kb on + strand, within PS417_11605 at 2496.424 kb on + strand, within PS417_11605 at 2496.425 kb on - strand, within PS417_11605 at 2496.745 kb on + strand, within PS417_11605 at 2496.746 kb on - strand, within PS417_11605 at 2496.746 kb on - strand, within PS417_11605 at 2496.895 kb on + strand, within PS417_11605 at 2497.111 kb on - strand, within PS417_11605 at 2497.201 kb on + strand, within PS417_11605 at 2497.201 kb on + strand, within PS417_11605 at 2497.202 kb on - strand, within PS417_11605 at 2497.234 kb on + strand, within PS417_11605 at 2497.303 kb on + strand, within PS417_11605 at 2497.802 kb on - strand, within PS417_11610 at 2497.949 kb on - strand, within PS417_11610
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 2,495,532 + PS417_11600 0.19 +0.1 2,495,532 + PS417_11600 0.19 +0.4 2,495,533 - PS417_11600 0.19 +0.9 2,495,580 + PS417_11600 0.24 -0.9 2,495,667 + PS417_11600 0.33 +1.2 2,495,668 - PS417_11600 0.33 -1.4 2,495,668 - PS417_11600 0.33 -0.3 2,496,070 - PS417_11600 0.77 +0.8 2,496,203 - -1.4 2,496,277 - +0.6 2,496,424 + +0.9 2,496,424 + PS417_11605 0.12 -0.5 2,496,424 + PS417_11605 0.12 -0.2 2,496,425 - PS417_11605 0.12 -0.8 2,496,745 + PS417_11605 0.39 -1.8 2,496,746 - PS417_11605 0.39 -0.2 2,496,746 - PS417_11605 0.39 -0.6 2,496,895 + PS417_11605 0.52 -0.5 2,497,111 - PS417_11605 0.71 +0.5 2,497,201 + PS417_11605 0.78 -0.2 2,497,201 + PS417_11605 0.78 -1.4 2,497,202 - PS417_11605 0.78 -0.1 2,497,234 + PS417_11605 0.81 -0.7 2,497,303 + PS417_11605 0.87 -0.7 2,497,802 - PS417_11610 0.47 +1.0 2,497,949 - PS417_11610 0.66 +0.5
Or see this region's nucleotide sequence