Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00110 and PS417_00115 are separated by 130 nucleotides
PS417_00110: PS417_00110 - tRNA threonylcarbamoyladenosine biosynthesis protein RimN, at 23,683 to 24,240
_00110
PS417_00115: PS417_00115 - quinone oxidoreductase, at 24,371 to 25,348
_00115
Position (kb)
23
24
25 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 22.863 kb on - strand at 23.317 kb on + strand at 23.318 kb on - strand at 23.318 kb on - strand at 23.401 kb on - strand at 23.627 kb on - strand at 23.665 kb on + strand at 24.364 kb on + strand at 24.364 kb on + strand at 24.364 kb on + strand at 24.364 kb on + strand at 24.365 kb on - strand at 24.365 kb on - strand at 24.365 kb on - strand at 24.365 kb on - strand at 24.365 kb on - strand at 24.365 kb on - strand at 24.366 kb on + strand at 24.397 kb on + strand at 24.526 kb on + strand, within PS417_00115 at 24.526 kb on + strand, within PS417_00115 at 24.527 kb on - strand, within PS417_00115 at 24.527 kb on - strand, within PS417_00115 at 24.543 kb on + strand, within PS417_00115 at 24.570 kb on + strand, within PS417_00115 at 24.571 kb on - strand, within PS417_00115 at 24.709 kb on - strand, within PS417_00115 at 24.942 kb on + strand, within PS417_00115 at 24.942 kb on + strand, within PS417_00115 at 24.942 kb on + strand, within PS417_00115 at 24.943 kb on - strand, within PS417_00115 at 24.943 kb on - strand, within PS417_00115 at 25.065 kb on + strand, within PS417_00115 at 25.212 kb on + strand, within PS417_00115 at 25.213 kb on - strand, within PS417_00115
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 22,863 - -1.6 23,317 + +0.3 23,318 - -0.5 23,318 - -2.5 23,401 - +0.1 23,627 - -0.4 23,665 + -2.2 24,364 + +0.2 24,364 + -1.0 24,364 + -1.8 24,364 + +0.3 24,365 - +0.4 24,365 - -2.7 24,365 - +0.0 24,365 - -3.0 24,365 - -0.0 24,365 - -0.8 24,366 + -1.8 24,397 + -0.3 24,526 + PS417_00115 0.16 -0.0 24,526 + PS417_00115 0.16 -2.0 24,527 - PS417_00115 0.16 +1.0 24,527 - PS417_00115 0.16 -0.5 24,543 + PS417_00115 0.18 +1.1 24,570 + PS417_00115 0.20 -0.5 24,571 - PS417_00115 0.20 -0.5 24,709 - PS417_00115 0.35 -0.5 24,942 + PS417_00115 0.58 -0.7 24,942 + PS417_00115 0.58 -1.9 24,942 + PS417_00115 0.58 +0.1 24,943 - PS417_00115 0.58 +1.5 24,943 - PS417_00115 0.58 +0.6 25,065 + PS417_00115 0.71 -0.7 25,212 + PS417_00115 0.86 -2.3 25,213 - PS417_00115 0.86 -0.8
Or see this region's nucleotide sequence