Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_09390 and PS417_09395 overlap by 4 nucleotides PS417_09395 and PS417_09400 overlap by 4 nucleotides PS417_09400 and PS417_09405 overlap by 4 nucleotides PS417_09405 and PS417_09410 are separated by 290 nucleotides
PS417_09390: PS417_09390 - hypothetical protein, at 2,036,082 to 2,036,642
_09390
PS417_09395: PS417_09395 - hypothetical protein, at 2,036,639 to 2,036,968
_09395
PS417_09400: PS417_09400 - thiamine pyrophosphate-binding protein, at 2,036,965 to 2,037,501
_09400
PS417_09405: PS417_09405 - peptidase, at 2,037,498 to 2,038,700
_09405
PS417_09410: PS417_09410 - isopropylmalate isomerase, at 2,038,991 to 2,040,409
_09410
Position (kb)
2037
2038
2039 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2036.591 kb on - strand at 2036.909 kb on - strand, within PS417_09395 at 2038.088 kb on + strand, within PS417_09405 at 2038.513 kb on + strand, within PS417_09405 at 2038.786 kb on - strand at 2038.786 kb on - strand at 2038.786 kb on - strand at 2038.786 kb on - strand at 2038.786 kb on - strand at 2038.864 kb on + strand at 2038.937 kb on + strand at 2039.009 kb on + strand at 2039.072 kb on + strand at 2039.072 kb on + strand at 2039.072 kb on + strand at 2039.072 kb on + strand at 2039.072 kb on + strand at 2039.073 kb on - strand at 2039.073 kb on - strand at 2039.074 kb on + strand at 2039.075 kb on - strand at 2039.075 kb on - strand at 2039.075 kb on - strand at 2039.075 kb on - strand at 2039.075 kb on - strand at 2039.076 kb on + strand at 2039.077 kb on - strand at 2039.077 kb on - strand at 2039.100 kb on - strand at 2039.148 kb on + strand, within PS417_09410 at 2039.253 kb on - strand, within PS417_09410 at 2039.400 kb on - strand, within PS417_09410 at 2039.400 kb on - strand, within PS417_09410
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 2,036,591 - +2.2 2,036,909 - PS417_09395 0.82 -1.1 2,038,088 + PS417_09405 0.49 +1.0 2,038,513 + PS417_09405 0.84 +2.0 2,038,786 - +0.7 2,038,786 - +0.6 2,038,786 - +0.4 2,038,786 - +0.8 2,038,786 - -0.7 2,038,864 + -2.4 2,038,937 + +1.2 2,039,009 + -1.1 2,039,072 + -1.6 2,039,072 + -0.2 2,039,072 + -0.1 2,039,072 + -0.9 2,039,072 + -0.9 2,039,073 - +0.0 2,039,073 - +0.9 2,039,074 + -0.1 2,039,075 - +0.3 2,039,075 - +0.4 2,039,075 - -1.8 2,039,075 - +1.0 2,039,075 - +0.9 2,039,076 + +1.1 2,039,077 - -1.2 2,039,077 - -1.1 2,039,100 - +0.4 2,039,148 + PS417_09410 0.11 -1.4 2,039,253 - PS417_09410 0.18 -1.9 2,039,400 - PS417_09410 0.29 -0.2 2,039,400 - PS417_09410 0.29 -0.5
Or see this region's nucleotide sequence