Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_08800 and PS417_08805 are separated by 165 nucleotides PS417_08805 and PS417_08810 are separated by 10 nucleotides PS417_08810 and PS417_08815 are separated by 256 nucleotides PS417_08815 and PS417_08820 are separated by 134 nucleotides
PS417_08800: PS417_08800 - tartronate semialdehyde reductase, at 1,914,782 to 1,915,672
_08800
PS417_08805: PS417_08805 - hydroxypyruvate isomerase, at 1,915,838 to 1,916,620
_08805
PS417_08810: PS417_08810 - glyoxylate carboligase (EC 4.1.1.47) (from data) , at 1,916,631 to 1,918,406
_08810
PS417_08815: PS417_08815 - GlcG protein, at 1,918,663 to 1,919,103
_08815
PS417_08820: PS417_08820 - transposase, at 1,919,238 to 1,919,696
_08820
Position (kb)
1916
1917
1918
1919 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1915.655 kb on - strand at 1915.697 kb on - strand at 1915.700 kb on + strand at 1915.700 kb on + strand at 1915.701 kb on - strand at 1915.701 kb on - strand at 1915.756 kb on - strand at 1915.900 kb on - strand at 1916.082 kb on + strand, within PS417_08805 at 1916.082 kb on + strand, within PS417_08805 at 1916.083 kb on - strand, within PS417_08805 at 1916.092 kb on - strand at 1916.160 kb on + strand, within PS417_08805 at 1916.160 kb on + strand, within PS417_08805 at 1916.160 kb on + strand, within PS417_08805 at 1916.160 kb on + strand at 1916.161 kb on - strand, within PS417_08805 at 1916.238 kb on + strand, within PS417_08805 at 1916.520 kb on + strand, within PS417_08805 at 1916.521 kb on - strand, within PS417_08805 at 1916.521 kb on - strand, within PS417_08805 at 1916.584 kb on + strand at 1916.620 kb on + strand at 1916.645 kb on - strand at 1916.726 kb on + strand at 1916.880 kb on + strand, within PS417_08810 at 1916.881 kb on - strand, within PS417_08810 at 1916.888 kb on - strand, within PS417_08810 at 1916.984 kb on - strand, within PS417_08810 at 1917.010 kb on + strand, within PS417_08810 at 1917.010 kb on + strand, within PS417_08810 at 1917.010 kb on + strand, within PS417_08810 at 1917.010 kb on + strand, within PS417_08810 at 1917.010 kb on + strand, within PS417_08810 at 1917.010 kb on + strand, within PS417_08810 at 1917.067 kb on + strand, within PS417_08810 at 1917.241 kb on + strand, within PS417_08810 at 1917.241 kb on + strand, within PS417_08810 at 1917.242 kb on - strand, within PS417_08810 at 1917.533 kb on - strand, within PS417_08810 at 1918.144 kb on + strand, within PS417_08810 at 1918.226 kb on - strand, within PS417_08810 at 1918.463 kb on + strand at 1918.463 kb on + strand at 1918.463 kb on + strand at 1918.463 kb on + strand at 1918.463 kb on + strand at 1918.463 kb on + strand at 1918.510 kb on + strand at 1918.539 kb on + strand at 1918.539 kb on + strand at 1918.539 kb on + strand at 1918.539 kb on + strand at 1918.540 kb on - strand at 1918.540 kb on - strand at 1919.023 kb on - strand, within PS417_08815 at 1919.119 kb on - strand at 1919.119 kb on - strand at 1919.171 kb on + strand at 1919.171 kb on + strand at 1919.172 kb on - strand at 1919.172 kb on - strand at 1919.172 kb on - strand at 1919.172 kb on - strand at 1919.172 kb on - strand at 1919.172 kb on - strand at 1919.183 kb on + strand at 1919.240 kb on - strand at 1919.317 kb on + strand, within PS417_08820 at 1919.317 kb on + strand, within PS417_08820 at 1919.317 kb on + strand, within PS417_08820 at 1919.318 kb on - strand, within PS417_08820 at 1919.318 kb on - strand, within PS417_08820 at 1919.387 kb on + strand, within PS417_08820
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 1,915,655 - +0.9 1,915,697 - -1.6 1,915,700 + -0.3 1,915,700 + -0.6 1,915,701 - -0.5 1,915,701 - -1.0 1,915,756 - -0.2 1,915,900 - -0.6 1,916,082 + PS417_08805 0.31 +1.2 1,916,082 + PS417_08805 0.31 +0.8 1,916,083 - PS417_08805 0.31 -0.3 1,916,092 - -0.2 1,916,160 + PS417_08805 0.41 +0.0 1,916,160 + PS417_08805 0.41 +0.1 1,916,160 + PS417_08805 0.41 -0.0 1,916,160 + +0.4 1,916,161 - PS417_08805 0.41 +0.1 1,916,238 + PS417_08805 0.51 +1.0 1,916,520 + PS417_08805 0.87 +0.6 1,916,521 - PS417_08805 0.87 -1.4 1,916,521 - PS417_08805 0.87 +0.7 1,916,584 + -2.0 1,916,620 + +1.4 1,916,645 - +0.0 1,916,726 + +1.4 1,916,880 + PS417_08810 0.14 -0.5 1,916,881 - PS417_08810 0.14 -1.5 1,916,888 - PS417_08810 0.14 -0.4 1,916,984 - PS417_08810 0.20 +0.7 1,917,010 + PS417_08810 0.21 -1.7 1,917,010 + PS417_08810 0.21 +0.4 1,917,010 + PS417_08810 0.21 -1.0 1,917,010 + PS417_08810 0.21 -1.4 1,917,010 + PS417_08810 0.21 -0.0 1,917,010 + PS417_08810 0.21 +0.4 1,917,067 + PS417_08810 0.25 -0.2 1,917,241 + PS417_08810 0.34 -1.5 1,917,241 + PS417_08810 0.34 -0.1 1,917,242 - PS417_08810 0.34 -1.5 1,917,533 - PS417_08810 0.51 +0.4 1,918,144 + PS417_08810 0.85 -2.1 1,918,226 - PS417_08810 0.90 -0.3 1,918,463 + -1.9 1,918,463 + -1.0 1,918,463 + +0.5 1,918,463 + +0.4 1,918,463 + -0.6 1,918,463 + +0.6 1,918,510 + -0.7 1,918,539 + +0.4 1,918,539 + +0.1 1,918,539 + +0.8 1,918,539 + -1.1 1,918,540 - +0.9 1,918,540 - -2.0 1,919,023 - PS417_08815 0.82 +1.7 1,919,119 - -1.4 1,919,119 - +0.8 1,919,171 + -0.8 1,919,171 + -0.7 1,919,172 - +0.5 1,919,172 - -0.6 1,919,172 - +2.2 1,919,172 - +2.2 1,919,172 - -0.5 1,919,172 - +1.7 1,919,183 + -0.8 1,919,240 - +0.1 1,919,317 + PS417_08820 0.17 -1.2 1,919,317 + PS417_08820 0.17 +1.0 1,919,317 + PS417_08820 0.17 +0.8 1,919,318 - PS417_08820 0.17 -0.9 1,919,318 - PS417_08820 0.17 +1.1 1,919,387 + PS417_08820 0.32 +0.0
Or see this region's nucleotide sequence