Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00060 and PS417_00065 are separated by 81 nucleotides PS417_00065 and PS417_00070 are separated by 40 nucleotides PS417_00070 and PS417_00075 are separated by 136 nucleotides PS417_00075 and PS417_00080 are separated by 17 nucleotides
PS417_00060: PS417_00060 - glycyl-tRNA synthetase, at 14,341 to 15,294
_00060
PS417_00065: PS417_00065 - 3-methyladenine DNA glycosylase, at 15,376 to 15,933
_00065
PS417_00070: PS417_00070 - lipid A biosynthesis lauroyl acyltransferase, at 15,974 to 16,861
_00070
PS417_00075: PS417_00075 - hypothetical protein, at 16,998 to 17,309
_00075
PS417_00080: PS417_00080 - potassium transporter peripheral membrane component, at 17,327 to 18,700
_00080
Position (kb) 15
16
17 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 15.309 kb on - strand at 15.375 kb on + strand at 15.412 kb on + strand at 15.412 kb on + strand at 15.412 kb on + strand at 15.421 kb on + strand at 15.422 kb on - strand at 15.524 kb on + strand, within PS417_00065 at 15.767 kb on + strand, within PS417_00065 at 16.856 kb on + strand at 16.924 kb on + strand at 16.924 kb on + strand at 16.925 kb on - strand at 17.079 kb on + strand, within PS417_00075 at 17.080 kb on - strand, within PS417_00075 at 17.165 kb on - strand, within PS417_00075 at 17.165 kb on - strand, within PS417_00075 at 17.268 kb on - strand, within PS417_00075 at 17.701 kb on - strand, within PS417_00080 at 17.701 kb on - strand, within PS417_00080 at 17.767 kb on + strand, within PS417_00080 at 17.767 kb on + strand, within PS417_00080 at 17.768 kb on - strand, within PS417_00080 at 17.768 kb on - strand, within PS417_00080 at 17.768 kb on - strand, within PS417_00080 at 17.768 kb on - strand, within PS417_00080 at 17.768 kb on - strand, within PS417_00080
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 15,309 - -1.5 15,375 + +0.2 15,412 + -1.0 15,412 + -1.9 15,412 + -1.0 15,421 + -0.5 15,422 - -0.8 15,524 + PS417_00065 0.27 -0.0 15,767 + PS417_00065 0.70 -2.1 16,856 + +0.5 16,924 + +0.3 16,924 + -0.1 16,925 - +1.3 17,079 + PS417_00075 0.26 +0.5 17,080 - PS417_00075 0.26 -0.6 17,165 - PS417_00075 0.54 +0.7 17,165 - PS417_00075 0.54 -0.2 17,268 - PS417_00075 0.87 +2.4 17,701 - PS417_00080 0.27 -2.8 17,701 - PS417_00080 0.27 -3.8 17,767 + PS417_00080 0.32 -0.8 17,767 + PS417_00080 0.32 -1.3 17,768 - PS417_00080 0.32 -1.9 17,768 - PS417_00080 0.32 +2.8 17,768 - PS417_00080 0.32 -0.0 17,768 - PS417_00080 0.32 -0.2 17,768 - PS417_00080 0.32 -0.2
Or see this region's nucleotide sequence