Strain Fitness in Pseudomonas simiae WCS417 around PS417_06655

Experiment: JP1 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_06645 and PS417_06650 are separated by 219 nucleotidesPS417_06650 and PS417_06655 overlap by 4 nucleotidesPS417_06655 and PS417_06660 overlap by 4 nucleotidesPS417_06660 and PS417_06665 are separated by 90 nucleotides PS417_06645: PS417_06645 - RNA polymerase-associated protein RapA, at 1,472,725 to 1,475,571 _06645 PS417_06650: PS417_06650 - aspartate-semialdehyde dehydrogenase, at 1,475,791 to 1,476,132 _06650 PS417_06655: PS417_06655 - class V aminotransferase, at 1,476,129 to 1,477,310 _06655 PS417_06660: PS417_06660 - endoribonuclease, at 1,477,307 to 1,477,777 _06660 PS417_06665: PS417_06665 - LysR family transcriptional regulator, at 1,477,868 to 1,478,734 _06665 Position (kb) 1476 1477 1478Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1475.318 kb on + strandat 1475.318 kb on + strandat 1475.319 kb on - strandat 1475.479 kb on + strandat 1475.603 kb on + strandat 1475.603 kb on + strandat 1475.603 kb on + strandat 1475.783 kb on + strandat 1475.783 kb on + strandat 1475.784 kb on - strandat 1475.790 kb on + strandat 1475.790 kb on + strandat 1475.790 kb on + strandat 1475.790 kb on + strandat 1475.790 kb on + strandat 1475.790 kb on + strandat 1475.791 kb on - strandat 1475.791 kb on - strandat 1475.791 kb on - strandat 1475.791 kb on - strandat 1475.791 kb on - strandat 1476.251 kb on - strand, within PS417_06655at 1476.380 kb on + strand, within PS417_06655at 1476.582 kb on + strand, within PS417_06655at 1476.631 kb on + strand, within PS417_06655at 1476.754 kb on + strand, within PS417_06655at 1476.755 kb on - strand, within PS417_06655at 1476.939 kb on + strand, within PS417_06655at 1476.939 kb on + strand, within PS417_06655at 1476.939 kb on + strand, within PS417_06655at 1476.940 kb on - strand, within PS417_06655at 1476.995 kb on + strand, within PS417_06655at 1476.995 kb on + strand, within PS417_06655at 1477.626 kb on + strand, within PS417_06660at 1477.626 kb on + strand, within PS417_06660at 1477.643 kb on + strand, within PS417_06660at 1477.643 kb on + strand, within PS417_06660at 1477.644 kb on - strand, within PS417_06660at 1477.644 kb on - strand, within PS417_06660at 1477.701 kb on + strand, within PS417_06660at 1477.701 kb on + strand, within PS417_06660at 1477.701 kb on + strand, within PS417_06660at 1477.701 kb on + strand, within PS417_06660at 1477.701 kb on + strand, within PS417_06660at 1477.702 kb on - strand, within PS417_06660at 1477.856 kb on + strandat 1478.050 kb on + strand, within PS417_06665at 1478.088 kb on + strand, within PS417_06665at 1478.089 kb on - strand, within PS417_06665at 1478.089 kb on - strand, within PS417_06665at 1478.133 kb on + strand, within PS417_06665at 1478.183 kb on + strand, within PS417_06665at 1478.184 kb on - strand, within PS417_06665at 1478.186 kb on + strand, within PS417_06665at 1478.186 kb on + strand, within PS417_06665at 1478.215 kb on + strand, within PS417_06665at 1478.215 kb on + strand, within PS417_06665at 1478.215 kb on + strand, within PS417_06665

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Per-strain Table

Position Strand Gene LocusTag Fraction JP1 MOI 1
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1,475,318 + +1.8
1,475,318 + +1.2
1,475,319 - -0.1
1,475,479 + -1.6
1,475,603 + -1.2
1,475,603 + -0.5
1,475,603 + +0.1
1,475,783 + +0.2
1,475,783 + -0.4
1,475,784 - -0.9
1,475,790 + +0.5
1,475,790 + +0.8
1,475,790 + +0.4
1,475,790 + +0.2
1,475,790 + +0.3
1,475,790 + +0.5
1,475,791 - +0.4
1,475,791 - +0.0
1,475,791 - -2.2
1,475,791 - +1.2
1,475,791 - +0.5
1,476,251 - PS417_06655 0.10 +1.5
1,476,380 + PS417_06655 0.21 +1.3
1,476,582 + PS417_06655 0.38 +0.3
1,476,631 + PS417_06655 0.42 -2.5
1,476,754 + PS417_06655 0.53 +0.4
1,476,755 - PS417_06655 0.53 +1.1
1,476,939 + PS417_06655 0.69 -1.4
1,476,939 + PS417_06655 0.69 -0.9
1,476,939 + PS417_06655 0.69 +1.5
1,476,940 - PS417_06655 0.69 -0.8
1,476,995 + PS417_06655 0.73 +1.2
1,476,995 + PS417_06655 0.73 -1.3
1,477,626 + PS417_06660 0.68 +1.7
1,477,626 + PS417_06660 0.68 -0.6
1,477,643 + PS417_06660 0.71 -1.0
1,477,643 + PS417_06660 0.71 -2.8
1,477,644 - PS417_06660 0.72 +0.1
1,477,644 - PS417_06660 0.72 -0.5
1,477,701 + PS417_06660 0.84 -0.8
1,477,701 + PS417_06660 0.84 -0.8
1,477,701 + PS417_06660 0.84 -2.4
1,477,701 + PS417_06660 0.84 +2.5
1,477,701 + PS417_06660 0.84 -1.3
1,477,702 - PS417_06660 0.84 -2.0
1,477,856 + +0.4
1,478,050 + PS417_06665 0.21 -0.2
1,478,088 + PS417_06665 0.25 +1.3
1,478,089 - PS417_06665 0.25 -1.0
1,478,089 - PS417_06665 0.25 -1.2
1,478,133 + PS417_06665 0.31 +1.5
1,478,183 + PS417_06665 0.36 +0.1
1,478,184 - PS417_06665 0.36 +2.4
1,478,186 + PS417_06665 0.37 -0.8
1,478,186 + PS417_06665 0.37 +0.5
1,478,215 + PS417_06665 0.40 +0.9
1,478,215 + PS417_06665 0.40 -0.2
1,478,215 + PS417_06665 0.40 -0.6

Or see this region's nucleotide sequence